Amplification and detection of transposon insertion flanking sequences using fluorescent MuAFLP

BioTechniques

Volumn 32, Issue 5, 2002, Pages 1090-1097

  Edwards, David ^a   Coghill, J ^a   Batley, J ^a   Holdsworth, M ^a   Edwards, K J ^a  

^a UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

AMPLIFICATION; FLUORESCENCE; GENES; PLANTS (BOTANY);

TRANSPOSON;

BIOTECHNOLOGY;

ARTICLE; DNA FLANKING REGION; FLUORESCENCE; GENE AMPLIFICATION; GENE INSERTION; GENE MUTATION; MAIZE; NONHUMAN; PLANT GROWTH; TRANSPOSON;

ZEA MAYS;

EID: 0036249290     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/02325rr04     Document Type: Article

References (10)

1. Characterization of the maize Mutator transposable element MURA transposase as a DNA-binding protein
2. A general method for gene isolation in tagging approaches: Amplification of insertion mutagenized sites (AIMS)
3. Identification of transposon tagged genes by the random sequencing of Mutator-tagged DNA fragments from Zea mays
4. DNA sequencing with Thermus-aquaticus DNA-polymerase and direct sequencing of polymerase chain reaction-amplified DNA
5. Mutator transposase is widespread in the grasses
6. Growing maize for genetic studies
7. Detection of specific sequences among DNA fragments separated by gel electrophoresis
8. Multiple independent defective Suppressor-Mutator transposon insertions in Arabidopsis: A tool for functional genomics
9. Solid-phase method for the purification of DNA sequencing reactions
10. Strategies for mutagenesis and gene cloning using transposon tagging and T-DNA insertional mutagenesis