Cloning, sequencing and overexpression of a Sinorhizobium meliloti M5N1CS carboxymethyl-cellulase gene

Applied Microbiology and Biotechnology

Volumn 58, Issue 6, 2002, Pages 767-771

  Michaud, P ^a   Belaich, A ^b   Courtois, B ^a   Courtois, J ^a  

^a UNIVERSITÉ DE PICARDIE JULES VERNE   (France)

^b CNRS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; BACTERIAL ENZYME; BACTERIAL PROTEIN; CARBOXYMETHYLCELLULASE; CARBOXYMETHYLCELLULOSE; CELLULASE; GLUCAN SYNTHASE; GLYCOSIDASE; UNCLASSIFIED DRUG;

GENE; MICROBIOLOGY;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; DNA ISOLATION; ENZYME ACTIVITY; ENZYME SUBSTRATE; ESCHERICHIA COLI; GENE OVEREXPRESSION; GENE SEQUENCE; ISOELECTRIC POINT; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; PROTEIN FAMILY; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; SINORHIZOBIUM MELILOTI;

BASE SEQUENCE; CELLULASE; CLONING, MOLECULAR; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENES, BACTERIAL; GLYCOSIDE HYDROLASES; SINORHIZOBIUM MELILOTI;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; SINORHIZOBIUM; SINORHIZOBIUM MELILOTI;

EID: 0036247453     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-002-0953-4     Document Type: Article

References (36)

1. Basic local alignment search tool
2. R factor transfer in Rhizobium leguminosarum
3. A (1→4)-β-D-glucuronan excreted by a mutant of the R. meliloti M5N1 strain
4. A novel method for efficient expression cloning of fungal enzymes genes
5. Characterization of Rhizobium leguminosarum exopolysaccharide glycanases that are secreted via type I exporter and have a novel heptapeptide repeat motif
6. Cloning of an Azorhizobium caulinodans endoglucanase gene and analysis of its role in symbiosis
7. Selection of a succinoglycan-deficient Rhizobium meliloti mutant producing a partially acetylated (1→4)-β-D-glucuronan. Symbiotic properties analysis
8. Studies on transformation of Escherichia coli with plasmids
9. Updating the sequence-based classification of glycoside hydrolases
10. CLUSTALW: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
11. Role of the two-components leader sequence and mature amino acid sequences in extracellular export of endoglucanase EGL from Pseudomonas solanacearum
12. Excretion of the egl gene product of Pseudomonas solanacearum
13. Regulation of pectinolysis in Erwinia Chrysanthemi
14. Cell-bound cellulase and polygalacturonase production by Rhizobium and Bradyrhizobium species
15. A cloned cellulase gene from Erwinia carotovora subsp. carotovora is expressed in Rhizobium fredii but does not influence nodulation of cowpea
16. Renaturation of enzyme after polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate
17. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
18. Degradative enzymes in Rhizobium meliloti
19. Structure and regulation of the Erwinia carotovora subspecies carotovora SCC3193 cellulase gene celVI and the role of cellulase in phytopathogenicity
20. Cell-associated pectinolytic and cellulolytic enzymes in Rhizobium leguminosarum biovar trifolii
21. Erosion of root epidermal cell walls by Rhizobium polysaccharide-degrading enzymes as related to primary host infection in the Rhizobium-legume symbiosis
22. Identification of a glucuronan lyase from a mutant strain of Rhizobium meliloti
23. MsPG3, a Medicago sativa polygalacturonase gene expressed during the alfalfa-Rhizobium meliloti interaction
24. A putative signal peptidase recognition site and sequence in eukaryotic and prokaryotic signals peptides
25. Polygalacturonase is a virulence factor in Agrobaterium tumefaciens biovar 3
26. Measurement of protein using bicinchoninic acid
27. Use of T7 RNA polymerase to direct expression of cloned genes
28. Use of Congo-red polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen
29. Auxin-induced release of cellulolytic activity from rhizobia and the promotion of effects of this activity on root hair curling in Avena sativa
30. The transcriptional activator XlnR regulates both xylanolytic and endoglucanase gene expression in Aspergillus niger
31. Assay of reducing sugars in the nanomole range with 2,2'-bicinchoninate
32. Structural characterization of the symbiotically important low-molecular-weight succinoglycan of Sinorhizobium meliloti
33. The Rhizobium meliloti ExoK and ExsH glycanases specifically depolymerize nascent succinoglycan chains
34. Extracellular glycanases of Rhizobium leguminosarum are activated on the cell surface by an exopolysaccharide-related component