Effects of intrinsic fluorescence and quenching on fluorescence-based screening of natural products

Phytomedicine

Volumn 9, Issue 3, 2002, Pages 263-267

  Zou, L ^a   Harkey, M R ^a   Henderson, G L ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Fluorescence based enzyme inhibition assay; Interference effects; Screening of natural products

Indexed keywords

6,7 DIHYDROXYBERGAMOTIN; ALLICIN; BERGAMOTIN; BILOBALIDE; CATECHIN; COMPLEMENTARY DNA; CYTOCHROME P450; DESMETHOXYANGONIN; DIHYDROKAWAIN; DIHYDROMETHYSTICIN; ESCIN; GINKGOLIC ACID I; GINKGOLIC ACID II; GINKGOLIDE A; GINKGOLIDE B; GINKGOLIDE C; HERBACEOUS AGENT; HYPERFORIN; ISORHAMNETIN; KAWAIN; LINOLEIC ACID; METHYSTICIN; NARINGENIN; NATURAL PRODUCT; QUERCETIN; SILIBININ; UNCLASSIFIED DRUG; UNINDEXED DRUG; VALERENIC ACID; VITEXIN; YANGONIN;

ANALYTIC METHOD; ANALYTICAL ERROR; ARTICLE; CONTROLLED STUDY; DRUG SCREENING; ENZYME ASSAY; ENZYME INHIBITION; FLUORESCENCE; FLUOROMETRY; PRIORITY JOURNAL;

EID: 0036245008     PISSN: 09447113     EISSN: None     Source Type: Journal    
DOI: 10.1078/0944-7113-00121     Document Type: Article

References (7)

1. Application of higher throughput screening (HTS) inhibition assays to evaluate the interaction of antiparasitic drugs with cytochrome P450s
2. An in vitro evaluation of human cytochrome P 450 3A4 inhibition by selected commercial herbal extracts and tinctures
3. Microtiter plate assays for inhibition of human, drug-metabolizing cytochromes P450
4. Use of cDNA-expressed human cytochrome P450 enzymes to study potential drug-drug interactions
5. Effects of ginseng components on c-DNA-expressed cytochrome P450 enzyme catalytic activity
6. Phenomenon of fluorescence