Demonstration and cytochemical analysis of anionic sites on ejaculated boar spermatozoa: A scanning electron microscopy study using cationised colloidal gold

Histochemistry and Cell Biology

Volumn 117, Issue 1, 2002, Pages 61-67

  Stoffel, Michael H ^a   Friess, Armin E ^a  

^a VETSUISSE FACULTY UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

Carboxyl groups; Cell cell interactions; Negative charges; Sialic acid

Indexed keywords

ANION; CARBOXYL GROUP; CATION; COLLOIDAL GOLD; PHOSPHATASE; PHOSPHOLIPID; PRONASE; SIALIC ACID;

AFFINITY LABELING; ANALYTIC METHOD; ANIMAL CELL; ARTICLE; BEAR; BINDING AFFINITY; CELL ADHESION; CONTROLLED STUDY; CYTOCHEMISTRY; EJACULATION; ELECTRON; HYDROLYSIS; MACROMOLECULE; METHYLATION; MOLECULAR RECOGNITION; NONHUMAN; PH; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; SPERMATOZOON; SURFACE CHARGE;

ACID PHOSPHATASE; ANIMALS; ANIONS; CATIONS; CELL MEMBRANE; GOLD COLLOID; HISTOCYTOCHEMISTRY; HYDROGEN-ION CONCENTRATION; MALE; MICROSCOPY, ELECTRON, SCANNING; NEURAMINIDASE; PRONASE; SEMEN; SPERMATOZOA; SWINE;

ANIMALIA; MAMMALIA;

EID: 0036244584     PISSN: 09486143     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00418-001-0350-6     Document Type: Article

References (39)

1. Identification of N- and O-linked oligosaccharides in the human epididymis
2. Changes in the electrophoretic properties of rabbit spermatozoa during passage through the epididymis
3. Drying cells for SEM, AFM and TEM by hexamethyldisilazane: A study on hepatic endothelial cells
4. Use of cationized ferritin as a label of negative charges on cell surfaces
5. Glycosaminoglycan-sulfate as plasma membrane component of pig spermatozoa
6. Ultrastructural and cytochemical modifications of rabbit spermatozoa during epididymal transport
7. Localization of glycoproteins and of negative and positive charges in the cell coat of ejaculated rabbit and bull spermatozoa (author's translation)
8. Ultrahistochemische untersuchungen an der glykokalyx von lymphozyten aus dem ductus thoracicus der ratte
9. Positive and negative colloidal iron as cell surface electron stains
10. Structure of the O-linked carbohydrate chains of porcine zona pellucida glycoproteins
11. Surface-bound sialic acid on ram and bull spermatozoa: Deposition during epididymal transit and stability during washing
12. Comparison of various bovine sperm capacitation systems for their ability to alter the net negative surface charge of spermatozoa
13. Cationic colloidal gold - A probe for light-microscope and electron-microscopic characterization of acidic glycoconjugates using poly-l-lysine gold complex
14. New insights into the origin, structure and role of Cd52: A major component of the mammalian sperm glycocalyx
15. N- and O-linked oligosaccharides in the secretory granules of rat Paneth cells: An ultrastructural cytochemical study
16. Cytochemical analysis of the anionic sites on the membrane of the stallion spermatozoa during the epididymal transit
17. The net surface charge of mammalian spermatozoa as determined by isoelectric focusing. Changes following sperm maturation, ejaculation, incubation in the female tract, and after enzyme treatment
18. Electrophoretic properties of bull and of rabbit spermatozoa
19. Anionic sites on the free surface of the peritoneal mesothelium: Light and electron microscopic detection using cationic colloidal iron
20. Characterization of the surface glycoproteins of rat spermatozoa
21. Histochemical application of mild alkaline hydrolysis for selective elimination of O-glycosidically linked glycoproteins
22. Detection of glycoconjugates in the ductus epididymis of the prepubertal and adult horse by lectin histochemistry
23. Charge interactions in sperm-egg recognition
24. Frozen boar spermatozoa: Methods of thawing pellets
25. Changes in the plasma membrane proteins of stallion spermatozoa during maturation in the epididymis
26. Acid and enzymatic hydrolysis of the internal sialic acid residue in native and chemically modified ganglioside GM1
27. Application of cationic colloidal gold staining for the demonstration of anionic sites
28. The glycocalyx of the sperm surface
29. Cationic colloidal gold: A new probe for the detection of anionic cell surface sites by electron microscopy
30. Histochemical characterization of anionic constituents in oocyte-cumulus complex of rats
31. An histochemical approach to characterization of anionic constituents in mast cell secretory granules
32. High-resolution scanning electron imaging of rat epididymal sperm after various aldehyde fixation protocols
33. Comparison of different drying procedures for scanning electron microscopy using human leukocytes
34. Anionic sites on Reissner's membrane, stria vascularis, and spiral prominence
35. Masking of sperm maturation antigen by sialic acid in the epididymis of the mouse. An immunohistochemical study
36. Quantitative detection of anionic sites in rat femoral cartilage using cationic colloidal gold at low pH levels
37. Interactions of macromolecules with the mammalian cell surface
38. The distribution of negative surface charges on mammalian spermatozoa