Tagging of Bacillus subtilis soil isolates through illegitimate recombination for PCR detection

World Journal of Microbiology and Biotechnology

Volumn 18, Issue 3, 2002, Pages 247-253

  Lara Reyna, Joel ^a   Olalde Portugal, Víctor ^b   Olmedo Alvarez, Gabriela ^a  

^a DEPARTAMENTO DE FÍSICA   (Mexico)

^b DEPARTAMENTO DE INGENIERÍA GENÉTICA   (Mexico)

Author keywords

Bacillus; Illegitimate recombination; PCR; Soil

Indexed keywords

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS);

EID: 0036239360     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1014915829586     Document Type: Article

References (21)

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3. Illegitimate recombination in Bacillus subtilis: Nucleotide sequences at recombinant DNA junctions
4. A rapid alkaline extraction procedure for screening recombinant plasmid DNA
5. Genetic analysis
6. Insertion of foreign DNA into plasmids from Gram-positive bacteria induces formation of high-molecular-weight plasmid multimers
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8. Integration of plasmid pE194 at multiple sites on the Bacillus subtilis chromosome
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13. Induction of DNA amplification in the Bacillus subtilis chromosome
14. Nomenclature for macrolide and macrolide-lincosamide-streptogramin B resistance determinants
15. Use of polymerase chain reaction and electroporation of Escherichia coli to monitor the persistence of extracellular plasmid DNA introduced into natural soils
16. Detection of specific sequences among the DNA fragments separated by gel electrophoresis
17. Replication control genes of plasmid pE194
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20. DNA recovery from soils of diverse composition