Effect of growth factors on ex vivo bone marrow cell expansion using three-dimensional matrix support

Artificial Organs

Volumn 26, Issue 4, 2002, Pages 333-339

  Tun, Thein ^a   Miyoshi, Hirotoshi ^a   Aung, Tun ^a   Takahashi, Satoru ^a   Shimizu, Ritsuko ^a   Kuroha, Takashi ^a   Yamamoto, Masayuki ^a   Ohshima, Norio ^a  

^a UNIVERSITY OF TSUKUBA   (Japan)

Author keywords

Bone marrow cells; Colony forming units; Growth factors; Hematopoietic progenitor cells; Polyvinyl formal; Three dimensional culture

Indexed keywords

BONE; RESINS; SCAFFOLDS (BIOLOGY); STEM CELLS;

BONE MARROW CELLS; CELL EXPANSION; COLONY FORMING UNITS; CULTURE SYSTEMS; EX-VIVO; GROWTH FACTOR; HEMATOPOIETIC PROGENITOR CELLS; POLYVINYL FORMALS; THREE DIMENSIONAL MATRICES; THREE-DIMENSIONAL CULTURE;

CELL PROLIFERATION;

ERYTHROPOIETIN; GROWTH FACTOR; INTERLEUKIN 3; INTERLEUKIN 6; POLYVINYL DERIVATIVE; RESIN; STEM CELL FACTOR;

ANIMAL CELL; ARTICLE; BONE MARROW CULTURE; CELL ASSAY; CELL CULTURE; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL STIMULATION; COLONY FORMING UNIT; CONTROLLED STUDY; CULTURE TECHNIQUE; HEMATOPOIETIC STEM CELL; INCUBATION TIME; MALE; METHODOLOGY; MOUSE; NONHUMAN; PRIORITY JOURNAL; TISSUE EXPANSION;

ANIMALIA; MURINAE;

EID: 0036238164     PISSN: 0160564X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1525-1594.2002.06842.x     Document Type: Article

References (30)

1. Enhanced maintenance and retroviral transduction of primitive hematopoietic progenitor cells using a novel three-dimensional culture system
2. Expansion in vitro of adult murine hematopoietic stem cells with transplantable lympho-myeloid reconstituting ability
3. Ex vivo expansion of murine hematopoietic progenitor cells generates classes of expanded cells possessing different levels of bone marrow repopulating potential
4. New type of matrix support for bone marrow cell cultures: In vitro culture and in vivo transplantation experiments
5. Soluble factor(s) produced by human bone marrow stroma increase cytokine-induced proliferation and maturation of primitive hematopoietic progenitors while preventing their terminal differentiation
6. Human osteoblasts support human hematopoietic progenitor cells in vitro bone marrow cultures
7. Stromal support enhances cell-free retroviral vector transduction of human bone marrow long-term culture-initiation cells
8. Bone marrow extracellular matrix molecules improve gene transfer into human hematopoietic cells via retroviral vectors
9. Packed-bed type reactor to attain high density culture of hepatocytes for use as a bioartificial liver
10. A packed-bed reactor utilizing porous resin enables high density culture of hepatocytes
11. Long-term continuous culture of hepatocytes in a packed-bed reactor utilizing porous resin
12. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assay
13. GATA factor transgenes under GATA-1 locus control rescue germline GATA-1 mutant deficiencies
14. GATA-1 regulates growth and differentiation of definitive erythroid lineage cells during in vitro ES cell differentiation
15. Restrictions in the stem cell function of murine bone marrow grafts after ex vivo expansion of short-term repopulating progenitors
16. Ex vivo expansion of murine marrow cells with interleukin-3 (IL-3), IL-6, IL-Il, and stem cell factor leads to impaired engraftment in irradiated hosts
17. Early hematopoietic and stromal precursor cells
18. Close range cell:cell interaction required for stem cell maintenance in continuous bone marrow culture
19. Conditions controlling the proliferation of hemopoietic stem cells in vitro
20. A three-dimensional culture system for the growth of hematopoietic cells
21. Multilineal hematopoiesis in a three-dimensional murine long-term bone marrow culture
22. Extended culture of multipotent hematopoietic progenitors without cytokine augmentation in a novel three-dimensional device
23. Engineering a human bone marrow model: A case study on ex vivo erythropoiesis
24. Ex vivo expansion of umbilical cord blood hematopoietic progenitor cells by combinations of cytokines
25. Maintenance of hematopoiesis in serum-free bone marrow cultures involves sequential recruitment of quiescent progenitors
26. + cells in serum-free cultures containing steel factor, interleukin-6, and erythropoietin with maintenance of cells with long-term in vivo reconstituting potential
27. Maintenance of transplantation potential in ex vivo expanded CD34+-selected human peripheral blood progenitor cells
28. Long-term culture-initiating cell expansion is dependent on frequent medium exchange combined with stromal and other accessory cell effects
29. Self-renewal of primitive human hematopoietic cells (longterm-culture-initiating cells) in vitro and their expansion in defined medium
30. Long-term expression of retrovirally introduced beta-galactosidase gene in rodent cells implanted in vivo using biodegradable polymer meshes