Characterization of the interaction of Bacillus subtilis PyrR with pyr mRNA by site-directed mutagenesis of the protein

Journal of Bacteriology

Volumn 184, Issue 9, 2002, Pages 2521-2528

  Savacool, Heather K ^a   Switzer, Robert L ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GENE PRODUCT; MESSENGER RNA; PROTEIN PYR; PROTEIN PYRR; PYRIMIDINE NUCLEOTIDE; UNCLASSIFIED DRUG; URACIL PHOSPHORIBOSYLTRANSFERASE;

AMINO ACID SEQUENCE; ARTICLE; BACILLUS SUBTILIS; BACTERIAL GENE; CONTROLLED STUDY; CRYSTALLOGRAPHY; GENE ACTIVITY; GENE CONTROL; GENE MUTATION; GENE STRUCTURE; IN VITRO STUDY; MISSENSE MUTATION; NONHUMAN; OPERON; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; RNA BINDING; RNA SEQUENCE; SITE DIRECTED MUTAGENESIS; TRANSCRIPTION REGULATION;

AMINO ACID SEQUENCE; BACILLUS SUBTILIS; BACTERIAL PROTEINS; BINDING SITES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PENTOSYLTRANSFERASES; PROTEIN BINDING; PROTEIN STRUCTURE, SECONDARY; REPRESSOR PROTEINS; RNA, BACTERIAL; RNA, MESSENGER; RNA-BINDING PROTEINS; SEQUENCE ALIGNMENT; TRANSCRIPTION, GENETIC;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); POSIBACTERIA; PROKARYOTA;

EID: 0036227921     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.184.9.2521-2528.2002     Document Type: Article

References (19)

1. Nutritional regulation of degradation of aspartate transcarbamylase and of bulk protein in exponentially growing Bacillus subtilis cells
2. Molecular recognition of pyr mRNA by the Bacillus subtilis attenuation regulatory protein, PyrR
3. Determination of the secondary structures of proteins by circular dichroism and optical rotatory dispersion
4. Themes in RNA-protein recognition
5. Characterization of cis-acting mutations in the first attenuator region of the Bacillus subtilis pyr operon that are defective in regulation of expression by pyrimidines
6. Mutations in Bacillus subtilis PyrR, the pyr regulatory protein with defects in regulation by pyrimidines
7. Regions of the Bacillus subtilis ilv-leu operon involved in regulation by leucine
8. The α-amylase gene as a marker for gene cloning: Direct screening of recombinant clones
9. Molscript. A program to produce both detailed and schematic plots of protein structures
10. Roles of the three transcriptional attenuators of the Bacillus subtilis pyrimidine biosynthetic operon in the regulation of its expression
11. Raster3D: Photorealistic molecular graphics
12. Modified methods for the determination of carbamyl aspartate
13. Plasmids designed to alter the antibiotic resistance expressed by insertion mutations in Bacillus subtilis through in vivo recombination
14. Regulation of the Bacillus subtilis pyrimidine biosynthetic operon by transcriptional attenuation: Control of gene expression by an mRNA-binding protein
15. Adaptation of an enzyme to regulatory function: Structure of Bacillus subtilis PyrR, a bifunctional pyr RNA-binding attenuation protein and uracil phosphoribosyltransferase
16. Purification and characterization of Bacillus subtilis PyrR, a bifunctional pyr mRNA-binding attenuation protein/uracil phosphoribosyltransferase
17. Regulation of the Bacillus subtilis pyrimidine biosynthetic (pyr) gene cluster by an autogenous transcriptional attenuation mechanism
18. Recent advances in PCR methodology