Residues near the N-terminus of protein B control autocatalytic proteolysis and the activity of soluble methane mono-oxygenase

European Journal of Biochemistry

Volumn 269, Issue 7, 2002, Pages 1835-1843

  Callaghan, Anastasia J ^a   Smith, Thomas J ^a   Slade, Susan E ^a   Dalton, Howard ^a  

^a UNIVERSITY OF WARWICK   (United Kingdom)

Author keywords

Autocatalytic inactivation; Methane mono oxygenase; Methanotroph; N terminal autoprocessing; Regulatory protein

Indexed keywords

AMINO ACID; BACTERIAL PROTEIN; COPPER; METHANE MONOOXYGENASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL CELL; BACTERIAL GROWTH; CATALYSIS; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME INACTIVATION; ENZYME PURIFICATION; ENZYME REGULATION; METHYLOCOCCUS CAPSULATUS; MOLECULAR WEIGHT; NONHUMAN; OXYGENATION; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN STABILITY;

BACTERIA (MICROORGANISMS); METHYLOCOCCUS; METHYLOCOCCUS CAPSULATUS;

EID: 0036227612     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1033.2002.02829.x     Document Type: Article

References (45)

1. Methanotrophic bacteria
2. Copper stress underlies the fundamental change in intracellular location of methane monooxygenase in methane-oxidizing organisms: Studies in batch and continuous cultures
3. Protein B of soluble methane monooxygenase from Methylococcus capsulatus (Bath): A novel regulatory protein of enzyme activity
4. Towards a unified mechanism of biological methane oxidation
5. Electron-spin-resonance studies of protein A of the soluble methane monooxygenase from Methylococcus capsulatus (Bath)
6. Crystal structure of a bacterial nonheme iron hydroxylase that catalyzes the biological oxidation of methane
7. Resolution of the methane monooxygenase of Methylococcus capsulatus (Bath) into three components
8. Electron-transfer reactions in the soluble methane monooxygenase of Methylococcus capsulatus (Bath)
9. Redox properties of the hydroxylase component of methane monooxygenase from Methylococcus capsulatus (Bath): Effects of protein B, reductase, and substrate
10. Direct electrochemistry of the hydroxylase of soluble methane monooxygenase from Methylococcus capsulatus (Bath)
11. Gating effects of component B on oxygen activation by the methane monooxygenase hydroxylase component
12. Kinetic and spectroscopic characterization of intermediates and component interactions in reactions of methane monooxygenase from Methylococcus capsulatus (Bath)
13. Mechanistic studies of the reaction of reduced methane monooxygenase hydroxylase with dioxygen and substrates
14. Activation of the hydroxylase of sMMO from Methylococcus capsulatus (Bath) by hydrogen peroxide
15. Methane monooxygenase component B and reductase alter the regioselectivity of the hydroxylase component-catalyzed reactions: A novel role for protein-protein interactions in an oxygenase mechanism
16. Inactivation of the regulatory protein B of soluble methane monooxygenase from Methylococcus capsulatus (Bath) by proteolysis can be overcome by a Gly to Gin modification
17. Component interactions in the soluble methane monooxygenase system from Methylococcus capsulatus (Bath)
18. Complex formation between the protein components of methane monooxygenase from Methylosinus trichosporium OB3b: Identification of sites of component interaction
19. Radiolytic reduction of methane monooxygenase dinuclear iron cluster at 77 K: EPR evidence for conformational change upon reduction or binding of component B to the diferric state
20. An EPR study of the dinuclear iron site in the soluble methane monooxygenase from Methylococcus capsulatus (Bath) reduced by one electron at 77 K: The effects of component interactions and the binding of small molecules to the diiron (III) center
21. Ligand field circular dichroism and magnetic circular dichroism studies of component B and substrate binding to the hydroxylase component of methane monooxygenase
22. Global conformational changes control the reactivity of methane monooxygenase
23. Structure of the soluble methane monooxygenase regulatory protein B
24. Crystal structures of the methane monooxygenase hydroxylase from Methylococcus capsulatus (Bath): Implications for substrate gating and component interactions
25. Crystal structure of the hydroxylase component of methane monooxygenase from Methylosinus trichosporium OB3b
26. Identification of the gene encoding the regulatory protein B of soluble methane monooxygenase
27. Mutational and structural analyses of the regulatory protein B of soluble methane monooxygenase from Methylococcus capsulatus (Bath)
28. Soluble methane monooxygenase from Methylococcus capsulatus Bath
29. An absolute method for protein determination based on differences of absorbance at 235 and 280nm
30. Measurement of protein by spectrophotometry at 205 nm
31. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of dye binding
32. Heat-tolerant methanotrophic bacteria from the hot-water effluent of a natural-gas field
33. Acetyl chymotrypsin and its reaction with ethanol
34. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
35. Breaking up is easy with esters
36. Breaking up is hard to do
37. Penicillin acylase has a single-amino-acid catalytic center
38. Activation of glycosylasparaginase: Formation of active N-terminal threonine by intramolecular autoproteolysis
39. Escherichia coli L-aspartate-α-decarboxylase: Preprotein processing and observation of reaction intermediates by electrospray mass spectrometry
40. Regulation of bacterial methane oxidation: Transcription of the soluble methane monooxygenase operon of Methylococcus capsulatus (Bath) is repressed by copper ions
41. Residues in Methylosinus trichosporium OB3b methane monooxygenase component B involved in molecular interactions with reduced- and oxidized-hydroxylase component: A role for the N-terminus
42. The alkene monooxygenase from Xanthobacter strain Py2 is closely related to aromatic monooxygenases and catalyzes aromatic monohydroxylation of benzene, toluene, and phenol
43. Purification and characterization of component B of a soluble methane monooxygenase from Methylocystis sp. M