Complications in cell-surface labelling by biotinylation of Candida albicans due to avidin conjugate binding to cell-wall proteins

Microbiology

Volumn 148, Issue 4, 2002, Pages 1073-1079

  Masuoka, James ^a   Guthrie, Lori N ^a   Hazen, Kevin C ^a  

^a UNIVERSITY OF VIRGINIA HEALTH SYSTEM   (United States)

Author keywords

Adhesion; Yeast

Indexed keywords

AVIDIN; BIOTIN; BIOTIN DERIVATIVE; CELL MEMBRANE PROTEIN; FUNGAL PROTEIN; LECTIN; SODIUM CHLORIDE; THIOCYANATE POTASSIUM;

ARTICLE; BIOTINYLATION; CANDIDA ALBICANS; CELL LABELING; CELL SURFACE; COMPLEX FORMATION; CONCENTRATION RESPONSE; CONJUGATE; CONTROLLED STUDY; HOST CELL; HYDROPHOBICITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN PROTEIN INTERACTION; WESTERN BLOTTING; YEAST;

CANDIDA; CANDIDA ALBICANS;

EID: 0036225809     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-148-4-1073     Document Type: Article

References (32)

1. Identification of a 49-kDa hydrophobic cell wall mannoprotein present in velum yeast which may be implicated in velum formation
2. Streptavidin contains an RYD sequence which mimics the RGD receptor domain of fibronectin
3. The use of the avidin-biotin complex as a tool in molecular biology
4. Enzyme-based detection of glycoproteins on blot transfers using avidin-biotin technology
5. Secular trends in the epidemiology of nosocomial fungal infections in the United States, 1980-1990
6. Characterization of cell wall proteins from yeast and mycelial cells of Candida albicans by labelling with biotin: Comparison with other techniques
7. Cell wall of Candida albicans: Its function and its impact on the host
8. Biotinylation: An alternative to radioiodination for the identification of cell surface antigens in immunoprecipitates
9. Prevention of non-specific binding of avidin
10. Cloning and expression of a gene encoding an integrin-like protein in Candida albicans
11. Solubilization of particulate proteins and nonelectrolytes by chaotropic agents
12. Optimal conditions for breaking medically important yeasts by an inexpensive and simple method
13. Temperature-modulated physiological characteristics of Candida albicans
14. Hydrophobic surface protein masking by the opportunistic fungal pathogen Candida albicans
15. Surface hydrophobic and hydrophilic protein alterations in Candida albicans
16. Partial biochemical characterization of cell surface hydrophobicity and hydrophilicity of Candida albicans
17. Identification of surface proteins on bovine leukocytes by a biotin-avidin protein blotting technique
18. Vitamins in milk. B. Water-soluble vitamins in bovine milk
19. Antibody directed to determinants of a Moloney virus derived MCF GP70 recognizes a thymic differentiation antigen
20. Evidence for the presence of Pir-like proteins in Candida albicans
21. A fibronectin receptor on Candida albicans mediates adherence of the fungus to extracellular matrix
22. Cleavage of structural proteins during assembly of the head of bacteriophage T4
23. Luminescent detection method for immunodot, Western and Southern blots
24. Hydrophobic and hydrophilic cell surface (glyco)proteinic components of Candida albicans
25. Specific labelling of cell wall proteins by biotinylation. Identification of four covalently linked O-mannosylated proteins of Saccharomyces cerevisiae
26. The collagen binding domain of fibronectin contains a high affinity binding site for Candida albicans
27. Structure of the Saccharomyces cerevisiae cell wall mannoproteins released by zymolyase and their contribution to wall architecture
28. Structure and function of the fungal cell wall
29. Cloning and analysis of a Candida albicans gene that affects cell surface hydrophobicity
30. The emerging fungal threat
31. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications