Close location of the first loop to the third loop of the mitochondrial ADP/ATP carrier deduced from cross-linking catalyzed by copper-o-phenanthroline of the solubilized carrier with Triton X-100

Journal of Biochemistry

Volumn 131, Issue 3, 2002, Pages 461-468

  Majima, Eiji ^a,b   Takeda, Masashi ^a   Miki, Satomi ^a   Shinohara, Yasuo ^a   Terada, Hiroshi ^a  

^a UNIVERSITY OF TOKUSHIMA   (Japan)

^b APRO Life Science Institute   (Japan)

Author keywords

ADP ATP carrier; Copper o phenanthroline; Cross linking; Intramolecular disulfide bridge; Mitochondria

Indexed keywords

1,10 PHENANTHROLINE COPPER; ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; PROTEINASE; TRITON X 100;

ARTICLE; CATALYSIS; CATALYST; CATTLE; CONFORMATION; CROSS LINKING; DISULFIDE BOND; HEART MITOCHONDRION; NONHUMAN; PROTEIN DEGRADATION;

BOS TAURUS; BOVINAE;

EID: 0036221428     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a003122     Document Type: Article

References (15)

1. Dialectics in carrier research the ADP/ATP carrier and the uncoupling protein
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3. Characterization of cysteine residues of mitochondrial ADP/ATP carrier with the SH-reagents eosin 5-maleimide and N-ethylmaleimide
4. Importance of loops of mitochondrial ADP/ATP carrier for its transport activity deduced from reactivities of its cysteine residues with the sulfhydryl reagent eosin-5-maleimide
5. Translocation of loops regulates transport activity of mitochondrial ADP/ATP carrier deduced from formation of a specific intermolecular disulfide bridge catalyzed by copper-o-phenanthroline
6. Fluctuation of the first loop facing the matrix of the mitochondrial ADP/ATP carrier deduced from intermolecular cross-linking of Cys56 residues by bifunctional dimaleimides
7. Important role of loops in the transport activity of the mitochondrial ADP/ATP carrier
8. Specific cardiolipin binding interferes with labeling of sulfhydryl residues in the adenosine diphosphate/adenosine triphosphate carrier protein from beef heart mitochondria
9. Reconstitution of ADP/ATP translocase in phospholipid vesicles
10. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
11. Topography of the membrane-bound ADP/ATP carrier assessed by enzymatic proteolysis
12. Dependence of the conformational state of the isolated adenine nucleotide carrier protein on the detergent used for solubilization
13. The reconstituted ADP/ATP carrier activity has an absolute requirement for cardiolipin as shown in cysteine mutants
14. Binding of the fluorescein derivative eosin Y to the mitochondrial ADP/ATP carrier: Characterization of the adenine nucleotide binding site
15. The transmembrane arrangement of the ADP/ATP carrier as elucidated by the lysine reagent pyridoxal 5-phosphate