Comparison of phbC genes cloned from Ralstonia eutropha and Alcaligenes latus for utilization in metabolic engineering of polyhydroxyalkanoate biosynthesis

Biotechnology Letters

Volumn 24, Issue 7, 2002, Pages 539-545

  Shin, Hyun Dong ^a   Oh, Dong Hyun ^a   Jung, Young Mi ^a   Ghim, Sa Youl ^a   Lee, Yong Hyun ^a  

^a Kyungpook National University   (South Korea)

Author keywords

Alcaligenes latus; Metabolic engineering; phbC gene; Polyhydroxy alkanoates; Ralstonia eutropha

Indexed keywords

POLYHYDROXYALKANOIC ACID;

ALCALIGENES LATUS; ARTICLE; BIOSYNTHESIS; CLONE; ENZYME ACTIVITY; GENE EXPRESSION; METABOLIC ENGINEERING; MUTANT; NONHUMAN; PLASMID; RALSTONIA EUTROPHA;

ALCALIGENES; ALCALIGENES LATUS; RALSTONIA; WAUTERSIA EUTROPHA;

EID: 0036220082     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1014899607533     Document Type: Article

References (23)

1. Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates
2. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
3. A rapid gas chromatographic method for the determination of poly-βhydroxybutyric acid in microbial biomass
4. Production of poly-D(-)-3-hydroxybutyrate and poly-D(-)-3-hydroxyvalerate by strains of Alcaligenes latus
5. Poly(3-hydroxybutyrate) production with high productivity and high polymer content by fed-batch culture of Alcaligenes latus under nitrogen limitation
6. Molecular cloning, sequencing and expression in Escherichia coli of the poly(3-hydroxyalkanoate) synthesis genes from Alcaligenes latus DSM1124
7. Production of poly(3-hydroxybutyrate-co-4-hydroxy butyrate) by Alcaligenes latus
8. Applications of PHB: A microbially produced biodegradable thermoplastic
9. Investigation of regulatory mechanism of flux of acetyl-CoA in Alcaligenes eutrophus using PHB negative mutant and transformants harboring cloned phbCAB genes
10. Metabolic engineering of Alcaligenes eutrophus through the transformation of cloned phbCAB genes for the investigation of the regulatory mechanism of polyhydroxyalkanoate biosynthesis
11. New bacterial copolyesters produced in Alcaligenes eutrophus from organic acids
12. Enhanced biosynthesis of P(3HB-3HV) and P(3HB-4HB) by amplification of the cloned PHB biosynthesis genes in Alcaligenes eutrophus
13. Construction of transformant Alcaligenes latus enforcing cloned own phbC gene and characterization of poly-β-hydroxy-buryrate biosynthesis
14. Analyses of a polyhydroxyalkanoic acid granule-associated 16-kilodalton protein and its putative regulator in the pha locus of Paracoccus denitrificans
15. Manipulation of the gene for poly-β-hydroxybutyric acid synthesis in Alcaligenes eutrophus
16. High frequency tranformation of Alcaligenes eutrophus producing polyβ-hydroxybutyric acid by electroporation
17. Production of poly-β-hydroxybutyrate by Alcaligenes eutrophus transformant harboring cloned phbCAB genes
18. Characteristics of cell growth and poly-β-hydroxybutyrate biosynthesis of Alcaligenes eutrophus transformants harboring cloned phbCAB genes
19. Poly-β-hydroxybutyrate biosynthesis in Alcaligenes eutrophus H16, identification and characterization of the PHB polymerase gene (phbC)
20. Cloning of the Alcaligenes eutrophus genes for synthesis of poly-β-hydroxybutyric acid (PHB) and synthesis of PHB in Escherichia coli
21. Production of P(3-hydroxybutyrate-3-hydroxy-valerate) and P(3-hydroxybutyrate-4-hydroxybutyrate) using transformant Alcaligenes latus enforcing cloned own phbC gene
22. Analysis of a 24-kilodalton protein associated with the polyhydroxyalkanoic acid granules in Alcaligenes eutrophus
23. Accumulation of the phaP phasin of Ralstonia eutropha is dependent on production of polyhydroxybutyrate in cells