A new GST-MAT vector containing both ipt and iaaM/H genes can produce marker-free transgenic tobacco plants with high frequency

Plant Cell Reports

Volumn 20, Issue 10, 2002, Pages 923-928

  Endo, S ^a   Kasahara, T ^a   Sugita, K ^a   Ebinuma, H ^a  

^a NIPPON PAPER INDUSTRIES CO   (Japan)

Author keywords

Indoleacetamide hydrolase gene; Marker free transgenic plant; Selectable marker; Sopentenyl transferase; Transgenic plant; Tryptophan monooxygenase gene

Indexed keywords

GENES; TISSUE; TOBACCO;

PLANT TISSUES;

PLANTS (BOTANY);

AGROBACTERIUM; EMBRYOPHYTA; NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM; RHIZOBIUM;

EID: 0036219824     PISSN: 07217714     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00299-001-0418-8     Document Type: Article

References (21)

1. T-DNA of Agrobacterium tumefaciens encodes an enzyme of cytokinin biosynthesis
2. The maternal chromosome set is the target of the T-DNA in the in planta transformation of Arabidopsis thaliana
3. Arabidopsis in planta transformation. Uses, mechanisms, and prospects for transformation of other species
4. Plant transformation: Problems and strategies for practical application
5. Female reproductive tissues are the primary target of Agrobacterium-mediated transformation by the Arabidopsis floral-dip method
6. Isolation of plant DNA from fresh tissue
7. MAT (multi-auto-transformation) vector system. The oncogenes of Agrobacterium as positive markers for regeneration and selection of marker-free transgenic plants
8. Selection of marker-free transgenic plants using the isopentenyl transferase gene
9. Principle of the MAT vector
10. Selection of marker-free transgenic plants using the oncogenes (ipt, rolA, B, C) of Agrobacterium as selectable markers
11. The isopentenyl transferase gene is effective as a selectable marker gene for plant transformation in tobacco (Nicotiana tabacum cv. Petite Havana SR1)
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14. Assaying chimeric genes in plants: The GUS gene fusion system
15. The effect of overproduction of two Agrobacterium tumefaciens T-DNA auxin biosynthetic gene products in transgenic petunia plants
16. A revised medium for rapid growth and bioassays with tobacco tissue cultures
17. Transgenic tobacco plants coexpressing Agrobacterium tumefaciens iaaM and iaaH metabolism
18. Cytokinin gene fused with a strong promoter enhanced shoot organogenesis and zeatin levels in transformed plant cells
19. Effective selection system for generating marker-free transgenic plants independent of sexual crossing
20. A transformation vector for the production of marker-free transgenic plants containing a single copy transgene at high frequency
21. Isolation and characterization of diverse nopaline type Ti plasmids of Agrobacterium tumefaciens from Japan