Acrylamide capture of DNA-bound complexes: Electrophoretic purification of transcription factors

BioTechniques

Volumn 32, Issue 4, 2002, Pages 808-815

  Nelson, Colleen ^a   Hendy, Stephen ^a   Reid, Kim ^a   Cavanagh, John ^a  

^a VANCOUVER GENERAL HOSPITAL   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING ENERGY; ELECTROPHORESIS; GELS; GENES; GENETIC ENGINEERING; POLYMERIZATION; PROTEINS;

ANDROGEN RECEPTORS;

DNA;

ACRYLAMIDE; ANDROGEN RECEPTOR; TRANSCRIPTION FACTOR;

AMINO TERMINAL SEQUENCE; ANALYTIC METHOD; ARTICLE; BINDING AFFINITY; BINDING SITE; DNA BINDING; ELECTROPHORESIS; GENE AMPLIFICATION; PROMOTER REGION; PROTEIN DNA INTERACTION; REGULATORY MECHANISM; RELIABILITY; TECHNIQUE; WESTERN BLOTTING;

EID: 0036213888     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/02324st07     Document Type: Article

References (10)

1. Identification and analysis of all components of a gel retardation assay by combination with immunoblotting
2. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei
3. Equilibria and kinetics of the lac repressor-operator interactions by polyacrylamide gel electrophoresis
4. A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: Application to components of the Escherichia coli lactose operon regulatory system
5. Affinity purification of sequence-specific DNA binding proteins
6. Mutation typing mobilized Acrydite probes
7. Two classes of androgen receptor elements mediate cooperativity through allosteric interactions
8. Mass spectrometric sequencing of proteins from silver-stained polyacrylamide gels
9. Β-catenin affects androgen receptor transcriptional activity and ligand specificity
10. Sequestration stabilizes lac repressor-DNA complexes during electrophoresis