Aggregation of human wild-type and H27A-prolactin in cells and in solution: Roles of Zn2+, Cu2+, and pH

Endocrinology

Volumn 143, Issue 4, 2002, Pages 1302-1309

  Sankoorikal, Binu John ^a   Yong Lian, Z H U ^a   Hodsdon, Michael E ^a   Lolis, Elias ^a   Dannies, Priscilla S ^a  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHLOROQUINE; COPPER ION; DIETHYLDITHIOCARBAMIC ACID; DIVALENT CATION; EDETIC ACID; LUBROL; PROLACTIN; PROPYLENE GLYCOL; ZINC ION;

ANIMAL CELL; ARTICLE; ATOMIC ABSORPTION SPECTROMETRY; BINDING SITE; CONTROLLED STUDY; ENDOPLASMIC RETICULUM; GOLGI COMPLEX; MOUSE; NEUROSECRETORY CELL; NONHUMAN; PH; PRECIPITATION; PRIORITY JOURNAL; PROTEIN AGGREGATION; SECRETORY GRANULE; SOLUBILITY;

EID: 0036212637     PISSN: 00137227     EISSN: None     Source Type: Journal    
DOI: 10.1210/endo.143.4.8732     Document Type: Article

References (55)

1. Molecular organization of rat prolactin granules. I. In vitro stability of intact and "membraneless" granules
2. Studies on the molecular organization of rat insulin secretory granules
3. Formation of secretory granules in the Golgi apparatus of prolactin cells in the rat pituitary gland: A stereoscopic study
4. Lack of correlation of distribution of prolactin (PRL) charge isoforms with induction of PRL storage
5. Acquisition of Lubrol insolubility, a common step for growth hormone and prolactin in the secretory pathway of neuroendocrine cells
6. Vesicles and cistemae in the trans Golgi apparatus of human fibroblasts are acidic compartments
7. Conversion of proinsulin to insulin occurs coordinately with acidification of maturing secretory vesicles
8. Direct measurement of trans-Golgi pH in living cells and regulation by second messengers
9. Mechanism of acidification of the trans-Golgi network (TGN). In situ measurements of pH using retrieval of TGN38 and furin from the cell surface
10. Mechanisms of pH regulation in the regulated secretory pathway
11. Carboxypeptidase E, a peripheral membrane protein implicated in the targeting of hormones to secretory granules, co-aggregates with granule content proteins at acidic pH
12. Calcium and pH-dependent aggregation of carboxypeptidase E
13. Secretory granule content proteins and the luminal domains of granule membrane proteins aggregate in vitro at mildly acidic pH
14. Milieu-induced, selective aggregation of regulated secretory proteins in the trans-Golgi network
15. Regulated secretory proteins in the exocrine pancreas aggregate under conditions that mimic the trans-Golgi network
16. Reconstitution in vitro of the pH-dependent aggregation of pancreatic zymogens en route to the secretory granule: Implication of GP-2
17. 1 cells
18. Secretory granule targeting of atrial natriuretic peptide correlates with its calcium-mediated aggregation
19. High-resolution calcium mapping of the endoplasmic reticulum-Golgi-exocytic membrane system. Electron energy loss imaging analysis of quick frozen-freeze dried PC12 cells
20. Interaction of calcium with porcine adrenal chromogranin A (secretory protein-I) and chromogranin B (secretogranin I)
21. Zinc in the anterior pituitary of rat: A histochemical and analytical work
22. ++
23. 2+-binding properties of bovine prolactin
24. Dimerizatlon of human growth hormone by zinc
25. Characterization of a peptide α-amidation activity from rat anterior pituitary
26. Secretory granules and granins in hyperstimulated male rat gonadotropes
27. The expression of cox17p in rodent tissues and cells
28. Low-molecular-weight constituents of isolated insulin-secretory granules. Bivalent cations, adenine nucleotides and inorganic phosphate
29. The influence of macromolecular crowding and macromolecular confinement on biochemical reactions in physiological media
30. Macromolecular crowding: An important but neglected aspect of the intracellular environment
31. Implications of macromolecular crowding for protein assembly
32. Bacterial production and purification of recombinant human prolactin
33. Biological activity and immunological reactivity of human prolactin mutants
34. Engineering human prolactin to bind to the human growth hormone receptor
35. 2+
36. The role of a low pH intracellular compartment in the processing, storage, and secretion of ACTH and endorphin
37. Primaquine blocks transport by inhibiting the formation of functional transport vesicles. Studies in a cell-free assay of protein transport through the Golgi apparatus
38. On the effects of weak bases and monensin on sorting and processing of lysosomal enzymes in human cells
39. The role of paired basic amino acids in mediating proteolytic cleavage of prosomatostatin. Analysis using site-directed mutagenesis
40. Inhibition of peptide amidation by disulfiram and diethyldithiocarbamate
41. Reaction of N,N-diethyldithiocarbamate and other bidentate ligands with Zn, Co and Cu bovine carbonic anhydrases. Inhibition of the enzyme activity and evidence for stable ternary enzyme-metal-ligand complexes
42. Metal binding modes of Alzheimer's amyloid β-peptide in insoluble aggregates and soluble complexes
43. Intracellular transport and packaging of prolactin: A quantitative electron microscope autoradiographic study of mammotrophs dissociated from rat pituitaries
44. Structural features of prolactins and growth hormones that can be related to their biological properties
45. Dramatic aggregation of Alzheimer αβ by Cu(II) is induced by conditions representing physiological acidosis
46. Binding of cadmium(II) and zinc(II) to human and dog serum albumins. An equilibrium dialysis and 113Cd-NMR study
47. Avoiding interferences from Good's buffers: A contiguous series of noncomplexing tertiary amine buffers covering the entire range of pH 3-11
48. Electrochemical evidence of surfactant activity of the hepes pH buffer which may have implications on trace metal availability to cultures in vitro
49. Free-radical generation by copper ions and hydrogen peroxide. Stimulation by Hepes buffer
50. Undetectable intracellular free copper: The requirement of a copper chaperone for superoxide dismutase
51. The concentration of twelve elements in the anterior pituitary from human subjects and rats as measured by particle induced X-ray emission (PIXE)
52. Colocalization of chaperone cpn60, proinsulin and convertase PC1 within immature secretory granules of insulin-secreting cells suggests a role for cpn60 in insulin processing
53. Protein hormone storage in secretory granules: Mechanisms for concentration and sorting