Optimisation of multiple PCR using a combination of full factorial design and three-dimensional simplex optimisation method

Biotechnology Letters

Volumn 24, Issue 6, 2002, Pages 489-495

  Benčina, Mojca ^a  

^a NATIONAL INSTITUTE OF CHEMISTRY   (Slovenia)

Author keywords

Full Factorial Design; Multiple PCR; RT PCR; Simplex optimisation method

Indexed keywords

DEOXYRIBONUCLEOTIDE; MAGNESIUM ION; PRIMER DNA;

ANALYTIC METHOD; ARTICLE; ASPERGILLUS NIGER; CONCENTRATION RESPONSE; CONTROLLED STUDY; FACTORIAL ANALYSIS; GENE AMPLIFICATION; INTERMETHOD COMPARISON; NONHUMAN; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TRANSCRIPTION REGULATION;

ASPERGILLUS; ASPERGILLUS NIGER;

EID: 0036206334     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1014502709890     Document Type: Article

References (16)

1. Non-radioactive multiple reverse transcription-PCR method used for low abundance mRNA quantification
2. Stress mediated changes in expression of the pkaC gene, encoding the catalytic subunit of cAMP-dependent protein kinase, in Aspergillus niger
3. Characterisation and overexpression of the Aspergillus niger gene encoding the cAMP-dependent protein kinase catalytic subunit
4. Experimental design: A useful tool for PCR optimisation
5. Optimisation via Simplex. Part I. Background, definitions and a simple application
6. Optimisation of multiplex PCRs
7. A simple procedure for optimizing the polymerase chain reaction (PCR) using modified Taguchi methods
8. Isolation and expression of the Aspergillus nidulans pyruvate kinase gene
9. Quantitative PCR: An overview
10. Cloning and characterisation of pepC, a gene encoding a serine protease from Aspergillus niger
11. Optimisation using the super-modified Simplex method
12. Comparison of primers and optimisation of PCR conditions for detection of Cryptosporidium parvum and Giardia lamblia in water
13. Enzymatic amplification of beta-globin genomic sequence and restriction site analysis for diagnosis of sickle cell anemia
14. The design and optimisation of the PCR
15. Optimisation strategies for the polymerase chain reaction