Efficient cloning of full-length cDNAs based on cDNA size fractionation

Genomics

Volumn 79, Issue 4, 2002, Pages 603-607

  Draper, Michael P ^a   August, Paul R ^a   Connolly, Timothy ^a   Packard, Brian ^b   Call, Katherine M ^a  

^a Transcept Pharmaceuticals Inc   (United States)

^b Massachussetts U   (United States)

Author keywords

cDNA; Cloning; Gene library; Genomics; mRNA

Indexed keywords

COMPLEMENTARY DNA;

ANALYTIC METHOD; ANIMAL CELL; ARTICLE; CONTAMINATION; DATA ANALYSIS; DNA LIBRARY; DNA MICROARRAY; GENE LIBRARY; GENE SEQUENCE; GENOME ANALYSIS; MOLECULAR BIOLOGY; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; START CODON; TISSUE SPECIFICITY;

ANIMALIA;

EID: 0036204051     PISSN: 08887543     EISSN: None     Source Type: Journal    
DOI: 10.1006/geno.2002.6738     Document Type: Article

References (20)

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3. Revolutions in rapid amplification of cDNA ends: New strategies for polymerase chain reaction cloning of full-length cDNA ends
4. Oligo-capping: A simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides
5. Construction of full-length-enriched cDNA libraries. The oligo-capping method
6. An efficient strategy to isolate full-length cDNAs based on an mRNA cap retention procedure (CAPture)
7. High-efficiency full-length cDNA cloning by biotinylated CAP trapper
8. Balanced-size and long-size cloning of full-length, cap-trapped cDNAs into vectors of the novel lambda-FLC family allows enhanced gene discovery rate and functional analysis
9. Normalization and subtraction of cap-trapper-selected cDNAs to prepare full-length cDNA libraries for rapid discovery of new genes
10. Amplification of cDNA ends based on template-switching effect and step- out PCR
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16. A simple and very efficient method for generating cDNA libraries
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19. The molecular evolution of actin
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