An update on conformation sensitive gel electrophoresis

Human Mutation

Volumn 19, Issue 4, 2002, Pages 334-342

  Ganguly, Arupa ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

BRCA1; BRCA2; CSGE; Mutation detection; Mutation scanning; SNP

Indexed keywords

1,4 BIS(ACROLYL)PIPERAZINE; CELL DNA; PIPERAZINE DERIVATIVE; UNCLASSIFIED DRUG;

CONFORMATION SENSITIVE GEL ELECTROPHORESIS; GEL ELECTROPHORESIS; GENE MUTATION; GENETIC SCREENING; HUMAN; ONCOGENE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REVIEW; SINGLE NUCLEOTIDE POLYMORPHISM;

BASE SEQUENCE; DNA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FLUORESCENCE; HETERODUPLEX ANALYSIS; MODELS, MOLECULAR; NUCLEIC ACID CONFORMATION; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL; SENSITIVITY AND SPECIFICITY;

EID: 0036199856     PISSN: 10597794     EISSN: None     Source Type: Journal    
DOI: 10.1002/humu.10059     Document Type: Review

References (26)

1. The contrasting structures of mismatched DNA sequences containing looped out bases (bulges) and multiple mismatches (bubbles)
2. A study of electrophoretic mobility of DNA in agarose and poly acrylamide gels
3. Superiority of denaturing high performance liquid chromatography over single-stranded conformation and conformation-sensitive gel electrophoresis for mutation detection in TSC2
4. Detection and location of single-base mutations in large DNA fragments by immunomicroscopy
5. Conformation-sensitive gel electrophoresis for rapid detection of single-base differences in double-stranded PCR products and DNA fragments: Evidence for solvent-induced bends in DNA heteroduplexes
6. High throughput fluorescence-based conformation-sensitive gel electrophoresis (F-CSGE) identifies six unique BRCA2 mutations and an overall low incidence of BRCA2 mutations in high-risk BRCA1-negative breast cancer families
7. Relationship between factor VIII mutation type and inhibitor development in a cohort of previously untreated patients treated with recombinant factor VIII (Recombinate)
8. Development of polyacrylamide gels that improve the separation of proteins and their detection by silver staining
9. Nucleosomal DNA sequence database
10. Rapid detection of single base mismatches as heteroduplexes on Hydrolink gels
11. DNA bending at adenine thymine tracts
12. Conformation sensitive gel electrophoresis for simple and accurate detection of mutations: Comparison with denaturing gradient gel electrophoresis and nucleotide sequencing
13. Use of conformation sensitive gel electrophoresis to detect single-base changes in the gene for COL10A1
14. Unwinding of double stranded DNA helix by dehydration
15. High-throughput conformation-sensitive gel electrophoresis for discovery of SNPs
16. Bending and flexibility of kinetoplast DNA
17. Physical characterization of a kinetoplast DNA fragment with unusual properties
18. A PCR artifact: Generation of heteroduplexes
19. DNA melting temperatures and renaturation rates in concentrated alkylammonium salt solutions
20. Hydrolink gels: A rapid and simple approach to the detection of DNA mutations in thromboembolic disease
21. Translational positioning of nucleosomes on DNA: The role of sequence-dependent isotropic DNA bending stiffness
22. Improved detection of mutations in the p53 gene in human tumors as single-stranded conformation polymorphs and double-stranded heteroduplex DNA
23. Detecting single base substitutions as heteroduplex polymorphisms
24. Three new point mutations in type II procollagen (COL2A1) and identification of a fourth family with the COL2A1 Arg519->Cys base substitution using conformation sensitive gel electrophoresis
25. The locus of sequence-directed and protein-induced DNA bending
26. DNA bend direction by phase sensitive detection