Binding of a pleckstrin homology domain protein to phosphoinositide in membranes: A miniaturized FRET-based assay for drug screening

Journal of Biomolecular Screening

Volumn 7, Issue 1, 2002, Pages 45-55

  Hamman, Brian D ^a   Pollok, Brian A ^a   Bennett, Todd ^a   Allen, Janet ^b   Heim, Roger ^a  

^a Aurora Biosciences Corporation   (United States)

^b Institut de Recherche Jouveinal   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BRUTON TYROSINE KINASE; GLUTATHIONE TRANSFERASE; GREEN FLUORESCENT PROTEIN; INOSITOL PHOSPHATE; PHOSPHATIDYLINOSITOL 3 PHOSPHATE; PHOSPHATIDYLINOSITOL 3,4 BISPHOSPHATE; PHOSPHATIDYLINOSITOL 3,4,5 TRISPHOSPHATE; PHOSPHATIDYLINOSITOL 4 PHOSPHATE; PHOSPHATIDYLINOSITOL 4,5 BISPHOSPHATE; PLECKSTRIN; POLYPHOSPHOINOSITIDE; PROTEIN TYROSINE KINASE; PROTEIN TYROSINE KINASE INHIBITOR; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; CONCENTRATION RESPONSE; DRUG SCREENING; ENZYME INHIBITOR INTERACTION; FLUORESCENCE ANALYSIS; FLUORESCENCE RESONANCE ENERGY TRANSFER; MOLECULAR INTERACTION; PHOSPHOLIPID VESICLE; PRIORITY JOURNAL; PROTEIN DOMAIN;

ANIMALS; BINDING, COMPETITIVE; BLOOD PROTEINS; CELL MEMBRANE; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG EVALUATION, PRECLINICAL; GLUTATHIONE TRANSFERASE; GREEN FLUORESCENT PROTEINS; HUMANS; KINETICS; LIPID METABOLISM; LUMINESCENT PROTEINS; MICE; MUTAGENESIS, SITE-DIRECTED; PHOSPHATIDYLINOSITOLS; PHOSPHOPROTEINS; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; PROTEIN-TYROSINE KINASES; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, FLUORESCENCE; TIME FACTORS;

EID: 0036197530     PISSN: 10870571     EISSN: None     Source Type: Journal    
DOI: 10.1089/108705702753520332     Document Type: Article

References (20)

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