Blue-white selection step enhances the yield of SAGE concatemers

BioTechniques

Volumn 32, Issue 3, 2002, Pages 484-486

  Angelastro, James M ^a   Ryu, E J ^a   Torocsik B ^a   Fiske, B K ^a   Greene, L A ^a  

^a Center for Neurobiology and Behavior ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA TOPOISOMERASE (ATP HYDROLYSING); HYBRID PROTEIN;

ARTICLE; CLONING VECTOR; ENZYME ACTIVITY; EXPRESSED SEQUENCE TAG; GENE EXPRESSION; GENETIC TRANSFECTION; MOLECULAR CLONING; NONHUMAN; SEQUENCE ANALYSIS;

UNIDENTIFIED CLONING VECTOR;

EID: 0036186555     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/02323bm02     Document Type: Article

References (12)

1. Improved NlaIII digestion of PAGE-purified 102 bp ditags by addition of a single purification step in both the SAGE and microSAGE protocols
2. Identification of diverse nerve growth factor-regulated genes by serial analysis of gene expression (SAGE) profiling
3. Bacterial death by DNA gyrase poisoning
4. Bacterial topoisomerases, anti-topoisomerases, and anti-topoisomerase resistance
5. Substantially enhanced cloning efficiency of SAGE (serial analysis of gene expression) by adding a heating step to the original protocol
6. Gene discovery using the serial analysis of gene expression technique: Implications for cancer research
7. Enhanced concatemer cloning-a modification to the SAGE (serial analysis of gene expression) technique
8. Genome and genetic resources from the Cancer Genome Anatomy Project
9. Genes expressed in human tumor endothelium
10. Serial analysis of gene expression
11. Characterization of the yeast transcriptome
12. Analysing uncharted transcriptomes with SAGE