Dual-function vector for protein expression in both mammalian cells and Xenopus laevis oocytes

BioTechniques

Volumn 32, Issue 3, 2002, Pages 536-540

  Jespersen, Thomas ^a   Grunnet, M ^a   Angelo, K ^a   Klaerke D A ^a   Olesen, S P ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; DNA; OPTIMIZATION; POTASSIUM; RNA;

PROTEIN EXPRESSION;

PROTEINS;

ION CHANNEL; POTASSIUM CHANNEL;

ANIMAL CELL; ARTICLE; CHO CELL; DNA VECTOR; EXPRESSION VECTOR; MAMMAL CELL; MOLECULAR CLONING; NONHUMAN; OOCYTE; PROTEIN EXPRESSION; RNA STABILITY; XENOPUS LAEVIS;

ANIMALIA; MAMMALIA; VECTORS; XENOPUS LAEVIS;

EID: 0036186510     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/02323st05     Document Type: Article

References (13)

1. Kr potassium channels with HERG and is associated with cardiac arrhythmia
2. A molecular basis for cardiac arrhythmia: HERG mutations cause Long QT syndrome
3. Preparation of RNA for injection into Xenopus oocytes
4. + channels
5. An analysis of 5′-noncoding sequences from 699 vertebrate messenger RNAs
6. Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs
7. The fate of foreign proteins introduced into Xenopus oocytes
8. High-level expression and detection of ion channels in Xenopus oocytes
9. The biosynthesis of biologically active proteins in mRNA microinjected Xenopus oocytes
10. Gating of Shaker K+ channels: I. Ionic and gating currents
11. KCNE2 confers background current characteristics to the cardiac KCNQ1 potassium channel
12. Ca channel cloned from myometrium