Identification and characterization of a pheromone 2 specific binding protein of Euplotes octocarinatus

European Journal of Protistology

Volumn 37, Issue 4, 2002, Pages 391-403

  Matthias, Möllenbeck ^a   Klaus, Heckmann ^b  

^a UNIVERSITY OF CONNECTICUT HEALTH CENTER   (United States)

^b UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

Euplotes octocarinatus; Gamone receptor interaction; Heterologous expression; Intercellular communication; Preconjugant interaction

Indexed keywords

CAENORHABDITIS; CAENORHABDITIS ELEGANS; CILIOPHORA; EUKARYOTA; EUPLOTES; EUPLOTES OCTOCARINATUS; HOMO; HOMO SAPIENS; MUS; MUS MUSCULUS; MUSCULUS; PROTOZOA; XENOPUS LAEVIS;

EID: 0036184068     PISSN: 09324739     EISSN: None     Source Type: Journal    
DOI: 10.1078/0932-4739-00804     Document Type: Article

References (59)

1. Trans-splicing of nuclear pre-mRNAs
2. Basic local alignment search tool
3. Transferrins: Insights into structure and function from studies on lactoferrin
4. The bonds that tie: Catalyzed disulfide bond formation
5. Two introns in the pheromone 3-encoding gene of Euplotes octocarinatus
6. Expression of the pheromone 3-encoding gene of Euplotes octocarinatus using a novel bacterial secretion vector
7. The pheromones and pheromone genes of new stocks of the Euplotes octocarinatus species complex
8. A method for the determination of the amino acid sequences in peptides
9. 32P-based hybridizations
10. Identification of cell surface polypeptides of the hypotrich ciliate Euplotes octocarinatus
11. Transformation of Paramecium by microinjection of a cloned serotype gene
12. Intra- and intercellular communication systems in ciliates
13. Description and application of an immunological detection system for analyzing glycoproteins on blots
14. Paarungssystem und genabhängige paarungstypdifferenzierung bei dem hypotrichen ciliaten Euplotes vannus OF Müller
15. Experimentelle untersuchungen an Euplotes crassus. I. Paarungssystem, konjugation und determination der paarungstypen
16. Über pheromone und pheromongene bei ciliaten: Auf dem weg zu einem molekularen verständnis der konjugation
17. Towards a molecular understanding of conjugation: On pheromomones and pheromone genes in Euplotes octocarinatus
18. Mating types and gamones in Euplotes octocarinatus
19. Mating types and mating inducing substances in Euplotes octocarinatus
20. Site-directed mutagenesis by overlap extension using the polymerase chain reaction
21. Non-radioactive labeling and detection of nucleic acids: I. A novel DNA labeling and detection system based on digoxigenin:anti-digoxigenin ELISA principle (digoxigenin system)
22. The nature and inheritance of mating types in Euplotes patella
23. Studies on the phosphorylation of myelin basic protein by protein kinase C and adenosine 3′:5′-monophosphate-dependent protein kinase
24. Mating type substances
25. Adolescence in Euplotes octocarinatus
26. The pheromones of the ciliate Euplotes octocarinatus not only induce conjugation but also function as chemoattractants
27. Gamone production in large-scale cultures of Euplotes octocarinatus
28. Electroblotting of multiple gels: A simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose
29. A simple method for displaying the hydrophatic character of a protein
30. The Edman degradation
31. Evidence that Euplotes raikovi is a gamone excreter ciliate
32. Evidence that the ciliate Euplotes raikovi releases mating inducing factors (gamones)
33. Mating pheromones
34. Ciliate pheromones
35. UGA is translated as cysteine in pheromone 3 of Euplotes octocarinatus
36. Pheromone 4 gene of Euplotes octocarinatus
37. Induction of conjugation union by cell-free fluid in the ciliate Blepharisma
38. Cell interaction by gamones in Blepharisma
39. Physiology and biochemistry of conjugation in ciliates
40. Fertilization and sexuality
41. Genetic relationship of 32 cell lines of the Euplotes octocarinatus species complex revealed by random amplified polymorphic DNA fingerprinting
42. Characterization of two genes encoding a fifth so far unknown pheromone of Euplotes octocarinatus
43. The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts
44. Mating types and mating inheritance in Euplotes minuta Yocom (Ciliata, Hypotrichida)
45. The autocrine mitogenic loop of the ciliate Euplotes raikovi: The pheromone membrane-bound forms are the cell binding sites and potential signaling receptors of soluble pheromones
46. Synthetic antibodies with a known three-dimensional structure
47. Conjugation in the ciliate Euplotes octocarinatus: Comparison of ciliary and cell body-associated glycoconjugates of nonmating-competent, mating-competent, and conjugating cells
48. Primary structure of Euplotes raikovi pheromones: Comparison of five sequences of pheromones from cells with variable mating interactions
49. Introduction to expression by fusion protein vectors
50. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
51. DNA sequencing with chain-termination inhibitors
52. The isolation of gamones 3 and 4 of Euplotes octocarinatus
53. Detection of specific sequences among DNA fragments separated by gel electrophoresis
54. Engineering Escherichia coli to secrete heterlogous gene products
55. The genes encoding pheromones 1 and 2 of Euplotes octocarinatus
56. Divergent seven transmembrane receptors are candidate chemosensory receptors in C. elegans
57. Some data on the conjugation of the ciliate Dileptus anser
58. Isolation and partial characterization of gamone 1 of Euplotes octocarinatus
59. Substrate specificity of protein kinase C. Use of synthetic peptides corresponding to physiological sites as probes for substrate recognition requirements