Purification and properties of an alkaline proteinase of Fusarium culmorum

European Journal of Biochemistry

Volumn 269, Issue 3, 2002, Pages 798-807

  Pekkarinen, Anja I ^a,b,c   Jones, Berne L ^c   Niku Paavola, Marja Leena ^b  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b VTT TECHNICAL RESEARCH CENTRE OF FINLAND   (Finland)

^c AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

Cereal; Chymotrypsin; Fungus; Proteinase; Subtilisin

Indexed keywords

ALKALINE PROTEINASE; ANILIDE; BENZYLSULFONYL FLUORIDE; BOWMAN BIRK INHIBITOR; CHYMOSTATIN; N SUCCINYLALANYLALANYLPROLYLLEUCINE 4 NITROANILIDE; N SUCCINYLALANYLALANYLPROLYLPHENYLALANINE 4 NITROANILIDE; SOYBEAN TRYPSIN INHIBITOR; SUBTILISIN; UNCLASSIFIED DRUG;

AMINO ACID ANALYSIS; AMINO ACID SEQUENCE; ARTICLE; AUTOLYSIS; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME STABILITY; FUSARIUM CULMORUM; GEL PERMEATION CHROMATOGRAPHY; GRAIN; HYDROLYSIS; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR WEIGHT; NONHUMAN; PH; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; TEMPERATURE DEPENDENCE;

AMINO ACID SEQUENCE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME ACTIVATION; ENZYME STABILITY; FUSARIUM; GELATIN; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; MOLECULAR SEQUENCE DATA; SEQUENCE HOMOLOGY, AMINO ACID; SERINE ENDOPEPTIDASES; SUBSTRATE SPECIFICITY; TEMPERATURE;

FUNGI; FUSARIUM; FUSARIUM CULMORUM; GLYCINE MAX;

EID: 0036180993     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.0014-2956.2001.02697.x     Document Type: Article

References (44)

1. Fusarium ear blight (scab) in small grain cereals - A review
2. Relationship between Fusarium infestation of barley and the gushing potential of malt
3. The effects of Fusarium graminearum infection on wheat kernels
4. Fusarium head blight: Effect of fungal proteases on wheat storage proteins
5. Changes in some biochemical components of wheat grain that was infected with Fusarium graminearum
6. Production of proteases by Fusarium species grown on barley grains and in media containing cereal proteins
7. Protease inhibitors in cereals
8. Protease inhibitors in plants: Genes for improving defenses against insects and pathogens
9. Simplified endoproteinase assays using gelatin or azogelatin
10. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
11. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
12. Enzymatic properties of alkaline proteinase from Aspergillus candidus
13. Purification and characterization of extracellular proteinases of Aspergillus oryzae
14. Isolation of alkaline and neutral proteases from Aspergillus flavus var. columnaris, a soy sauce koji mold
15. Purification and characterization of a fibrinolytic serine proteinase from Aspergillus fumigatus culture filtrate
16. Production, purification and general properties of Fusarium alkaline protease
17. Production of an alkaline protease by Fusarium graminearum grown on whey
18. The sequence and X-ray structure of the trypsin from Fusarium oxysporum
19. The purification and activities of an alkaline protease of Aspergillus clavatus from Nigerian poultry feeds
20. Chymotrypsinogens-chymotrypsins
21. Trypsinogens and trypsins of various species
22. Fungal proteinase expression in the interaction of the plant pathogen Magnaporthe poae with its host
23. Families of serine peptidases
24. Solid surface-catalyzed inactivation of bovine α-chymotrypsinindilutesolution
25. Cloning and nucleotide sequences of the complementary and genomic DNAs for the alkaline protease from Acremonium chrysogenum
26. Cloning and characterization of the pepD gene of Aspergillus niger which codes for a subtilisin-like protease
27. Cloning and characterisation of pepC, a gene encoding a serine protease from Aspergillus niger
28. Isolation and characterization of an Aspergillus nidulans gene encoding an alkaline protease
29. Nucleotide sequence of a genomic and a cDNA clone encoding an extracellular alkaline protease of Aspergillus fumigatus
30. Isolation, characterization, and cloning of cDNA and the gene for an elastinolytic serine proteinase from Aspergillus flavus
31. Characterization of a novel allergen, a major IgE-binding protein from Aspergillus flavus, as an alkaline serine protease
32. A full length cDNA clone for the alkaline protease from Aspergillus oryzae: Structural analysis and expression in Saccharomyces cerevisiae
33. Molecular characterization of the proteinase-encoding gene, prb1, related to mycoparasitism by Trichoderma harzianum
34. Isolation of a cDNA encoding a novel subtilisin-like protease (Pr1B) from the entomopathogenic fungus, Metarhizium anisopliae using differential display-RT-PCR
35. Isolation and characterization of the gene encoding a novel, thermostable serine proteinase from the mould Tritirachium album Limber
36. Proteinase K from Tritirachium album Limber. Characterization of the chromosomal gene and expression of the cDNA in Escherichia coli
37. Cloning and nucleotide sequence of the alkaline protease gene from Fusarium sp. S-19-5 and expression in Saccharomyces cerevisiae
38. Cloning and sequencing of the alkaline extracellular protease gene of Yarrowia lipolytica