Molecular cloning of an extracellular aspartic proteinase from Rhizopus microsporus and evidence for its expression during infection

Medical Mycology

Volumn 40, Issue 1, 2002, Pages 61-71

  Schoen, C ^a,b   Reichard, U ^a   Monod, M ^c   Kratzin, H D ^d   Ruuchel R ^a  

^a UNIVERSITY OF GÖTTINGEN   (Germany)

^b UNIVERSITY OF WÜRZBURG   (Germany)

^c LAUSANNE UNIVERSITY HOSPITAL   (Switzerland)

^d MAX PLANCK INSTITUTE OF EXPERIMENTAL MEDICINE   (Germany)

Author keywords

Aspartic proteinase (E.C.3.4.23.21); Mucormycosis; Rhizopus microsporus

Indexed keywords

AGAROSE; ASPARTIC PROTEINASE; BLOOD CLOTTING FACTOR 10; FUNGAL DNA; PEPSTATIN; RECOMBINANT ENZYME;

ACIDITY; AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BRAIN INFECTION; COMPARATIVE STUDY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME CHEMISTRY; ENZYME PURIFICATION; ENZYME SYNTHESIS; EXTRACELLULAR FLUID; FEMALE; FUNGAL GENE; FUNGAL STRAIN; FUNGUS CULTURE; FUNGUS ISOLATION; HUMAN; IMMUNE RESPONSE; IN VITRO STUDY; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR CLONING; MUCORMYCOSIS; NONHUMAN; NORTHERN BLOTTING; PH; PICHIA PASTORIS; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN INDUCTION; RHIZOPUS; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; SOUTHERN BLOTTING; SUPERNATANT;

AMINO ACID SEQUENCE; ANIMALS; ASPARTIC ENDOPEPTIDASES; CLONING, MOLECULAR; GUINEA PIGS; HUMANS; MOLECULAR SEQUENCE DATA; MUCORMYCOSIS; RHIZOPUS;

BOVINAE; FUNGI; MICROSPORUS; MYXOGASTRIA; PICHIA; PICHIA PASTORIS; RHIZOPUS; RHIZOPUS MICROSPORUS; RHIZOPUS MICROSPORUS VAR. RHIZOPODIFORMIS; RHIZOPUS NIVEUS;

EID: 0036180541     PISSN: 13693786     EISSN: None     Source Type: Journal    
DOI: 10.1080/mmy.40.1.61.71     Document Type: Article

References (47)

1. Mucorales
2. Mucormycosis
3. Mucormycosis
4. Mucormycosis (zygomycosis): Is there news for the clinician?
5. Rhinocerebral and systemic mucormycosis. Clinical experience with 36 cases
6. Zygomycetes in human disease
7. Invasive gastrointestinal zygomycosis in a liver transplant recipient: Case report and review of zygomycosis in solid-organ transplant recipients
8. Zygomycosis in HIV-positive patients: A review of the literature
9. Mucormycosis in patients with haematological malignancies: A retrospective clinical study of 37 cases
10. Deferoxamine therapy and mucormycosis in dialysis patients: Report of an international registry
11. Effect of ferrioxamine on the growth of Rhizopus
12. Mould biodiversity in homes I. Air and surface analysis of 130 dwellings
13. Zygomycosis of the respiratory tract
14. Extracellular proteolysis by Mucoraceae in serum-albumin agar tested by the agar block method
15. Rhinocerebral mucormycosis during deferoxamine treatment
16. The expression of the secreted aspartyl proteinases Sap4 to Sap6 from Candida albicans in murine macrophages
17. Purification and characterization of an extracellular aspartic proteinase from Aspergillus fumigatus
18. Ion exchange chromatography
19. Cleaving of structural proteins during the assembly of the head of bacteriophage T4
20. Gas-phase sequencing after electroblotting on polyvinylidene difluoride membranes assigns correct molecular weights to myoglobin molecular weight markers
21. On the renin-like activity of Candida proteinases and activation of blood coagulation in vitro
22. Transformation by integration in Aspergillus nidulans
23. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease
24. DNA sequencing with chain-terminating inhibitors
25. Sequencing reactions for the Applied Biosystems 373A Automated DNA sequencer
26. Isolation and sequencing of a genomic clone encoding aspartic proteinase of Rhizopus niveus
27. Amino acid sequence of rhizopuspepsin isozyme p15
28. Determination of the amino acid sequences of the two major isoenzymes of Rhizopuspepsin
29. A second gene for a secreted aspartate proteinase in Candida albicans
30. Molecular cloning and sequencing of the gene encoding an extracellular aspartic proteinase from Aspergillus fumigatus
31. Primary structure of human pepsinogen gene
32. A structural comparison of 21 inhibitor complexes of the aspartic proteinase from Endothia parasitica
33. Purification and characterization of rhizopuspepsin isoenzymes from a liquid culture of Rhizopus chinensis
34. The Rhizopus oryzae secreted aspartic proteinase gene family: An analysis of gene expression
35. Molecular and biotechnological aspects of microbial proteinases
36. Gastrointestinal aspergillosis and zygomycosis in cattle
37. Proteolytic enzymes of Penicillium janthinellum. I. Purification and properties of a trypsinogen activating enzyme (peptidase A)
38. Activation of blood clotting factors by microbial proteinases
39. The structure and organisation of nuclear genes of filamentous fungi
40. A new method for predicting signal sequence cleavage sites
41. The structure and function of the aspartic proteinases
42. Activation of the plasma kallikrein-kinin system by Candida albicans proteinase
43. Role of aspartic proteases in disseminated Candida albicans infection in mice
44. Virulence of an aspergillo-pepsin-deficient mutant of Aspergillus fumigatus and evidence for another aspartic proteinase linked to the fungal cell wall
45. In vitro and in vivo anticandidal astivity of human immunodeficiency protease inhibitors
46. Influence of the HIV-proteinase inhibitor Saquinavir and Indinavir on the in vitro activity of secreted aspartyl proteinases from Candida albicans isolates of HIV-infected patients