An improved PCR-based megaprimer method for site-directed mutagenesis

Chinese Journal of Medical Genetics

Volumn 19, Issue 1, 2002, Pages 68-71

  Mo, Qiuhua ^a   Xu, Xiangmin ^a   Zhong, Xionglin ^a   Liu, Zhongying ^a  

^a SOUTHERN MEDICAL UNIVERSITY   (China)

Author keywords

globin gene; Megaprimer; Polymerase chain reaction; Site directed mutagenesis

Indexed keywords

CLONING; DNA;

PLASMIDS;

MUTAGENESIS;

PLASMID DNA;

ARTICLE; BETA THALASSEMIA; BINDING SITE; CHINESE; CLONE; DNA FLANKING REGION; DNA TEMPLATE; DNA VECTOR; GENE MUTATION; HUMAN; MOLECULAR CLONING; POLYMERASE CHAIN REACTION; SITE DIRECTED MUTAGENESIS;

ASIAN CONTINENTAL ANCESTRY GROUP; BETA-THALASSEMIA; DNA PRIMERS; GLOBINS; HUMANS; MUTAGENESIS, SITE-DIRECTED; POLYMERASE CHAIN REACTION;

EID: 0036179183     PISSN: 10039406     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (11)

1. Rapid insertional mutagenesis of DNA by polymerase chain reaction (PCR)
2. The "megaprimer" method of site-directed mutagenesis
3. Simple version of "megaprimer" PCR for site-directed mutagenesis
4. A one-step polymerase chain reaction site-directed mutagenesis method for large gene-cssettes with high efficiency, yield, and fidelity
5. Molecular study and prenatal diagnosis of alph- and beta-thalassemias in Chinese
6. A simple approach to prenatal diagnosis of β thalassemia
7. Site-directed mutagenesis using overlap extension PCR
8. Approaches to DNA mutagenesis: An overview
9. PCR amplification of up to 35-kb DNA with high fidelity and high yield from bacteriophage templates
10. PCR fidelity of Pfu DNA polymerase and other thermostable DNA polymerase