Analysis of ftsQ mutant alleles in Escherichia coli: Complementation, septal localization, and recruitment of downstream cell division proteins

Journal of Bacteriology

Volumn 184, Issue 3, 2002, Pages 695-705

  Chen, Joseph C ^a   Minev, Michael ^a   Beckwith, Jon ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MEMBRANE PROTEIN; PROTEIN FTSQ; UNCLASSIFIED DRUG;

ALLELE; ARTICLE; CELL DIVISION; ESCHERICHIA COLI; GENE MUTATION; GENETIC ANALYSIS; GENETIC COMPLEMENTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0036176969     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.184.3.695-705.2002     Document Type: Article

References (40)

1. The bimodular G57-V577 polypeptide chain of the class B penicillin-binding protein 3 of Escherichia coli catalyzes peptide bond formation from thiolesters and does not catalyze glycan chain polymerization from the lipid II intermediate
2. FtsN, a late recruit to the septum in Escherichia coli
3. Membrane topology of penicillinbinding protein 3 of Escherichia coli
4. Towards single-copy gene expression systems making gene cloning physiologically relevant: Lambda InCh, a simple Escherichia coli plasmid-chromosome shuttle system
5. Localization of cell division protein FtsQ by immunofluorescence microscopy in dividing and nondividing cells of Escherichia coli
6. The FtsQ protein of Escherichia coli: Membrane topology, abundance, and cell division phenotypes due to overproduction and insertion mutations
7. FtsQ, FtsL and FtsI require FtsK, but not FtsN, for co-localization with FtsZ during Escherichia coli cell division
8. Septal localization of FtsQ, an essential cell division protein in Escherichia coli
9. The proper ratio of FtsZ to FtsA is required for cell division to occur in Escherichia coli
10. Cloning and characterization of ftsN, an essential cell division gene in Escherichia coli isolated as a multicopy suppressor of tsA12(Ts)
11. Topological characterization of the essential Escherichia coli cell division protein FtsN
12. Membrane topology of the N-terminus of the Escherichia coli FtsK division protein
13. Direct random mutagenesis of gene-sized DNA fragments using polymerase chain reaction
14. Cell division in Escherichia coli: Role of FtsL domains in septal localization, function, and oligomerization
15. Localization of FtsL to the Escherichia coli septal ring
16. FtsL, an essential cytoplasmic membrane protein involved in cell division in Escherichia coli
17. BAD promoter
18. Domain-swapping analysis of FtsI, FtsL, and FtsQ, bitopic membrane proteins essential for cell division in Escherichia coli
19. Conservation of the 168 divIB gene in Bacillus subtilis W23 and B. licheniformis, and evidence for homology to ftsQ of Escherichia coli
20. Peptidoglycan synthetic enzyme activities of highly purified penicillin-binding protein 3 in Escherichia coli: A septum-forming reaction sequence
21. Membrane-bound division proteins DivIB and DivIC of Bacillus subtilis function solely through their external domains in both vegetative and sporulation division
22. PCR-based random mutagenesis using manganese and reduced dNTP concentration
23. 2 regulatory elements
24. Themes and variations in prokaryotic cell division
25. Identification of the mpl gene encoding UDP-N-acetylmuramate: L-alanyl-γ-D-glutamylmeso-diaminopimelate ligase in Escherichia coli and its role in recycling of cell wall peptidoglycan
26. The structure and function of Escherichia coli penicillin-binding protein 3
27. Conservation of gene order amongst cell wall and cell division genes in Eubacteria, and ribosomal genes in Eubacteria and eukaryotic organelles
28. An in vivo pathway for disulfide bond isomerization in Escherichia coli
29. Bacterial cell division
30. DivIB, FtsZ and cell division in Bacillus subtilis
31. A novel rho promoter::Tn10 mutation suppresses an ftsQ1(Ts) missense mutation in an essential Escherichia coli cell division gene by a mechanism not involving polarity suppression
32. An Escherichia coli mutation preventing degradation of abnormal periplasmic proteins
33. Characterization of degP, a gene required for proteolysis in the cell envelope and essential for growth of Escherichia coli at high temperature
34. Characterization of the ftsZ gene from Mycoplasma pulmonis, an organism lacking a cell wall
35. Localization of FtsI (PBP3) to the septal ring requires its membrane anchor, the Z ring, FtsA, FtsQ, and FtsL