A novel ISFET-type biosensor based on P450 monooxygenases

Biosensors and Bioelectronics

Volumn 17, Issue 3, 2002, Pages 173-179

  Hara, Masayuki ^a   Yasuda, Yoshiaki ^b   Toyotama, Hideki ^b   Ohkawa, Hideo ^c   Nozawa, Tsunenori ^d   Miyake, Jun ^a  

^a NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^b STANLEY ELECTRIC CO LTD   (Japan)

^c KOBE UNIVERSITY   (Japan)

^d TOHOKU UNIVERSITY   (Japan)

Author keywords

Biosensor; CYP1A1; Dichlorophenol; Ethoxycoumarin; ISFET; P450

Indexed keywords

CATALYST ACTIVITY; ELECTRIC POTENTIAL; ENZYME IMMOBILIZATION; ENZYME INHIBITION; FLUORESCENCE; GENETIC ENGINEERING; ION SENSITIVE FIELD EFFECT TRANSISTORS; PH SENSORS; YEAST;

MONOOXYGENASES;

BIOSENSORS;

7 ETHOXYCOUMARIN; CHLOROPHENOL; CYTOCHROME P450 1A1; CYTOCHROME P450 INHIBITOR; HYBRID PROTEIN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE FERRIHEMOPROTEIN REDUCTASE; UMBELLIFERONE;

ARTICLE; CATALYSIS; CHEMICAL MODIFICATION; CONTROLLED STUDY; CORRELATION ANALYSIS; ELECTRIC POTENTIAL; ELECTROCHEMICAL ANALYSIS; ELECTRONIC SENSOR; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME MECHANISM; ENZYMIC BIOSENSOR; FLUORESCENCE; MICROSOME MEMBRANE; NONHUMAN; PROTEIN EXPRESSION; SACCHAROMYCES CEREVISIAE; SEMICONDUCTOR;

BIOSENSING TECHNIQUES; CHLOROPHENOLS; CYTOCHROME P-450 CYP1A1; ELECTROCHEMISTRY; HYDROGEN-ION CONCENTRATION; MANGANESE;

SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0036165436     PISSN: 09565663     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0956-5663(01)00252-4     Document Type: Article

References (19)

1. A biomarker, P450RGS, for assessing the induction potential of environmental samples
2. Field effect transistor sensitive to penicillin
3. Microtiter plate assays for inhibition of human, drug-metabolizing cytochrome P450
4. On a new enzyme transducer combination: The enzyme transistor
5. A microsonsor for adenosine-5′-triphosphate pH-sensitive field effect transisters
6. Regeneration of NADPH by cactus chloroplasts: Coupling reaction with P450 monooxygenase
7. Immobilization of P450 monooxygenase and chloroplast for use in light-driven bioreactors
8. Uncoupling of the cytochrome P-450cam monooxygenase reaction by a single mutation, threonin-252 to alanine or valine: A possible role of the hydroxy amino acid in oxygen activation
9. Micro-biosensor
10. Resolution of the cytochrome P-450-containing ω-hydroxylation system of liver microsomes into three components
11. The carbon monooxide-binding pigment of liver microsomes
12. Kinetic studies on a genetically engineered fused enzyme between rat cytochrome P4501A1 and yeast NADPH-P450 reductase
13. Organelle electrode
14. Coupled enzyme reactions in enzyme electrodes using sequence, amplification, competition and antiinterference principles
15. Ah receptor, a novel ligand-activated transcription factor
16. Substrate specificity of camphor-induced cytochrome P-450 immobilized on an electrode
17. Inhibitors of cytochrome P-450 and their mechanism of action