A non-radioactive protein truncation test for the sensitive detection of all stop and frameshift mutations

Human Mutation

Volumn 19, Issue 2, 2002, Pages 165-172

  Kahmann, Sabine ^a   Herter, Peter ^a   Kuhnen, Cornelius ^b   Müller, Klaus Michael ^b   Muhr, Gert ^b   Martin, Dirk ^b   Soddemann, Matthias ^a   Müller, Oliver ^a  

^a MAX PLANCK INSTITUTE OF MOLECULAR PHYSIOLOGY   (Germany)

^b RUHR UNIVERSITY BOCHUM   (Germany)

Author keywords

Adenomatous polyposis coli; APC; Hybrid capture; In vitro synthesis protein assay; IVSP; Mutation detection; Protein truncation test; PTT; Tumor suppressor

Indexed keywords

APC PROTEIN;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; COLON POLYPOSIS; COLORECTAL CANCER; DILUTION; FRAMESHIFT MUTATION; GENE SEQUENCE; GENETIC ANALYSIS; GENETIC TRANSCRIPTION; HUMAN; HUMAN TISSUE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN TRUNCATION TEST; RNA TRANSLATION; SEQUENCE ANALYSIS; TECHNIQUE; TUMOR SUPPRESSOR GENE; WESTERN BLOTTING;

ADENOMATOUS POLYPOSIS COLI; ADENOMATOUS POLYPOSIS COLI PROTEIN; BASE SEQUENCE; BLOTTING, WESTERN; CODON; CODON, NONSENSE; DNA; DNA MUTATIONAL ANALYSIS; FECES; FRAMESHIFT MUTATION; GENES, APC; GENETIC SCREENING; HUMANS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; POLYMERASE CHAIN REACTION; PROTEIN BIOSYNTHESIS; RADIOISOTOPES; SENSITIVITY AND SPECIFICITY; TEMPLATES, GENETIC; TRANSCRIPTION, GENETIC;

EID: 0036161944     PISSN: 10597794     EISSN: None     Source Type: Journal    
DOI: 10.1002/humu.10024     Document Type: Article

References (10)

1. The protein truncation test: A review
2. Detection of APC mutations in stool DNA of patients with colorectal cancer by HD-PCR
3. A new method for detecting DNA mutations
4. Molecular diagnosis of familial adenomatous polyposis
5. Protein truncation test (PTT) for rapid detection of translating-terminating mutations
6. Introduction of a myc reporter tag to improve the quality of mutation detection using the protein truncation test
7. Nucleic acid-based methods for the detection of cancer
8. Rapid detection of translation-terminating mutations at the adenomatous polyposis coli (APC) gene by direct protein truncation test