Chemostat approach for the directed evolution of biodesulfurization gain-of-function mutants

Applied and Environmental Microbiology

Volumn 68, Issue 2, 2002, Pages 691-698

  Arensdorf, Joseph J ^a   Loomis, A Katrina ^a   DiGrazia, Philip M ^a   Monticello, Daniel J ^a   Pienkos, Philip T ^a  

^a Enchira Biotechnology Corporation   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CHEMOSTATS; ENZYMES; GENES; SULFUR;

MUTANTS;

MICROBIOLOGY;

BACTERIAL DNA; BENZOTHIOPHENE DERIVATIVE; ORGANOSULFUR DERIVATIVE; SULFIDE; THIOPHENE DERIVATIVE;

CHEMOSTAT;

ARTICLE; BACTERIAL GROWTH; DESULFURIZATION; DNA SEQUENCE; ENZYME ACTIVITY; EVOLUTION; GENE MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; RHODOCOCCUS; STRAIN DIFFERENCE;

BASE SEQUENCE; CULTURE MEDIA; EVOLUTION, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; OXIDOREDUCTASES; OXYGENASES; RHODOCOCCUS; SUBSTRATE SPECIFICITY; SULFIDES; SULFUR COMPOUNDS; THIOPHENES;

BACTERIA (MICROORGANISMS); RHODOCOCCUS; RHODOCOCCUS ERYTHROPOLIS;

EID: 0036156586     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.68.2.691-698.2002     Document Type: Article

References (34)

1. Directed evolution of biocatalysts
2. Emulsification of crude oil by an alkane-oxidizing Rhodococcus species isolated from seawater
3. New technologies for high-throughput screening
4. DNA shuffling method for generating highly recombined genes and evolved enzymes
5. Kinetic studies of pigment synthesis by non-sulfur purple bacteria
6. Sulfur compounds in fossil fuels
7. Characterization of the desulfurization genes from Rhodococcus sp. strain IGTS8
8. Selection in chemostats
9. Microbial desulfurization of alkylated dibenzothiophenes from a hydrodesulfurized middle distillate by Rhodococcus erythropolis I-19
10. Lysine-overproducing mutants of Saccharomyces cerevisiae baker's yeast isolated in continuous culture
11. Isolation of a unique benzothiophene-desulphurizing bacterium, Gordona sp. strain 213E (NCIMB 40816), and characterization of the desulphurization pathway
12. Molecular mechanisms of biocatalytic desulfurization of fossil fuels
13. Operon structure and functional analysis of the genes encoding thermophilic desulfurizing enzymes of Paenibacillus sp. A11-2
14. Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti
15. Utilization of organosulphur compounds by axenic and mixed cultures of Rhodococcus rhodochrous IGTS8
16. Desulfurization of coal: The microbial solution
17. Kinetic analysis of microbial desulfurization of model and light gas oils containing multiple alkyl dibenzothiophenes
18. Desulfurization of alkylated forms of both dibenzothiophene and benzothiophene by a single bacterial strain
19. A review of the occurrence, toxicity, and biodegradation of condensed thiophenes found in petroleum
20. A new method of designing hybrid genes - The homolog recombination method
21. Genetic analysis of the dsz promoter and associated regulatory regions of Rhodococcus erythropolis IGTS8
22. Alkylated benzothiophene desulfurization by Rhodococcus sp. strain T09
23. Biodesulfurization and the upgrading of petroleum distillates
24. Desulphurisation of benzothiophene and dibenzothiophene by actinomycete organisms belonging to the genus Rhodococcus, and related taxa
25. High-throughput screening of enzyme libraries
26. Sequence and molecular characterization of a DNA region encoding the dibenzothiophene desulfurization operon of Rhodococcus sp. strain IGTS8
27. DNA sequencing with chain-terminating inhibitors
28. Enzyme evolution in Rhodobacter sphaeroides: Selection of a mutant expressing a new galactitol dehydrogenase and biochemical characterization of the enzyme
29. DNA shuffling by random fragmentation and reassembly: In vitro recombination for molecular evolution
30. Rapid evolution of a protein in vitro by DNA shuffling
31. Selection in chemostat culture of a mutant strain of Clostridium tyrobutyricum improved in its reduction of ketones
32. Use of a glycerol-limited, long-term chemostat for isolation of Escherichia coli mutants with improved physiological properties
33. Molecular evolution by staggered extension process (StEP) in vitro recombination