In vitro selection of a deoxyribozyme that can utilize multiple substrates

Journal of Molecular Evolution

Volumn 54, Issue 2, 2002, Pages 180-190

  Levy, Matthew ^a   Ellington, Andrew D ^a  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

Deoxyribozyme; In vitro selection; Ligase; Nucleic acid replicator; Origins of life

Indexed keywords

DEOXYRIBOZYME; HEXANUCLEOTIDE; LIGASE; NUCLEOTIDE DERIVATIVE; OLIGONUCLEOTIDE; PHOSPHOTHIOESTER; THIOESTER; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; ENZYME KINETICS; ENZYME SUBSTRATE COMPLEX; GENE REPLICATION; GENETIC LINKAGE; LIGATION; PROTEIN SECONDARY STRUCTURE; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY;

ANIMALS; BASE SEQUENCE; CONSERVED SEQUENCE; DNA, CATALYTIC; KINETICS; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; OLIGONUCLEOTIDES; SELEX APTAMER TECHNIQUE; SEQUENCE DELETION; SUBSTRATE SPECIFICITY;

EID: 0036148792     PISSN: 00222844     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00239-001-0066-1     Document Type: Article

References (29)

1. Isolation of new ribozymes from a large pool of random sequences
2. A DNA enzyme that cleaves RNA
3. In vitro selection of self-cleaving DNAs
4. A DNA metalloenzyme with DNA ligase activity
5. The secondary structure and sequence optimization of an RNA ligase ribozyme
6. Structurally complex and highly active RNA ligases derived from random RNA sequences
7. Experimental testing of theories of an early RNA world
8. Ribozyme selection
9. The scene of a frozen accident
10. Evidence for the metal-cofactor independence of an RNA phosphodiester-cleaving DNA enzyme
11. The RNA world
12. Isolation of novel ribozymes that ligate AMP-activated RNA substrates
13. Catalysis of RNA cleavage by the Tetrahymena thermophila ribozyme. 1. Kinetic description of the reaction of an RNA substrate complementary to the active site
14. Approaches to solid phase DNA sequencing
15. The fidelity of template-directed oligonucleotide ligation and the inevitability of polymerase function
16. Chiral selection in poly(C)-directed synthesis of oligo(G)
17. The early evolution of the genetic code
18. Rhyme or reason: RNA-arginine interactions and the genetic code
19. Section of deoxyribozyme ligases that catalyze the formation of an unnatural internucleotide linkage
20. Expanded sequence dependence of thermodynamic parameters improves prediction of RNA secondary structure
21. The origin of polynucleotide-directed protein synthesis
22. In vitro selection of an allosteric ribozyme that transduces analytes to amplicons
23. A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics
24. The improbability of prebiotic nucleic acid synthesis
25. On the origin of RNA splicing and introns
26. Self-replication of complementary nucleotide-based oligomers
27. Coding coenzyme handles: A hypothesis for the origin of the genetic code
28. Evolution of coenzymes and the origin of pryidine nucleotides
29. A novel 5′-iodonucleoside allows efficient nonenzymic ligation of single-stranded and duplex