Expression of human microsomal epoxide hydrolase in Saccharomyces cerevisiae reveals a functional role in aflatoxin B1 detexification

Toxicological Sciences

Volumn 65, Issue 1, 2002, Pages 35-42

  Kelly, Edward J ^a   Erickson, Kristin E ^a   Sengstag, Christian ^b   Eaton, David L ^a  

^a UNIVERSITY OF WASHINGTON   (United States)

^b ETH ZURICH   (Switzerland)

Author keywords

Aflatoxin B1; Cytochrome P450; Genotocity; Microsomal epoxide hydrolase; Saccharomyces cerevisiae

Indexed keywords

AFLATOXIN B1; CARCINOGEN; COMPLEMENTARY DNA; CYTOCHROME P450 1A1; CYTOCHROME P450 1A2; EPOXIDE HYDROLASE; MICROSOME ENZYME; MYCOTOXIN;

AMES TEST; ARTICLE; BIOTRANSFORMATION; CARCINOGENICITY; CHEMICAL ANALYSIS; CONTROLLED STUDY; DETOXIFICATION; DNA ADDUCT; ENZYME KINETICS; GENE CONVERSION; GENE EXPRESSION; GENE LOCUS; GENETIC ENGINEERING; GENOTOXICITY; MITOTIC RECOMBINATION; MUTAGENICITY; NONHUMAN; PROTEIN EXPRESSION; PROTEIN FUNCTION; SACCHAROMYCES CEREVISIAE;

AFLATOXIN B1; CYTOCHROME P-450 CYP1A1; CYTOCHROME P-450 CYP1A2; DNA ADDUCTS; EPOXIDE HYDROLASES; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HUMANS; MICROSOMES; MUTAGENICITY TESTS; ORGANISMS, GENETICALLY MODIFIED; PLASMIDS; SACCHAROMYCES CEREVISIAE;

EUKARYOTA; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0036147887     PISSN: 10966080     EISSN: None     Source Type: Journal    
DOI: 10.1093/toxsci/65.1.35     Document Type: Article

References (32)

1. Catalytic triad of microsomal epoxide hydrolase: Replacement of Glu404 with Asp leads to a strongly increased turnover rate
2. 1-8,9-epoxide conjugating activities of two bacterially expressed α class glutathione S-transferase isozymes from mouse and rat
3. 1 in isolated rat hepatocytes
4. 1-induced cytotoxicity and inhibition of RNA synthesis in isolated rat hepatocytes
5. A case-control study of microsomal epoxide hydrolase, smoking, meat consumption, glutathione S-transferase M3, and risk of colorectal adenomas
6. Aflatoxin toxicology
7. 1, defines a subfamily of aldo-keto reductases
8. Saccharomyces cerevisiae: An alternative source for human microsomal liver enzymes and its use in drug interaction studies
9. Heterologous expression of human microsomal epoxide hydrolase in Saccharomyces cerevisiae. Study of the valpromide-carbamazepine epoxide interaction
10. Constitutive and inducible expression of human cytochrome P450IA1 in yeast Saccharomyces cerevisiae: An alternative enzyme source for in vitro studies
11. 1
12. Radiometric assays for mammalian epoxide hydrolases and glutathione S-transferase
13. Human cytochrome P450 enzymes
14. 1 and relevance to risk of human liver cancer
15. Human microsomal epoxide hydrolase: Genetic polymorphism and functional expression in vitro of amino acid variants
16. 1 in the rat
17. 1 exo-8,9-oxide and rearrangement of the dihydrodiol
18. 1 exo-8,9-epoxide catalyzed by rat and human glutathione transferases: Estimation of kinetic parameters
19. Aflatoxin B1 8,9-epoxide hydrolysis in the presence of rat and human epoxide hydrolase
20. 1
21. 1 in human-derived in vitro systems
22. Epoxide hydrolase-polymorphism and role in toxicology
23. The carbon monoxide-binding pigment of liver microsomes: II. Solubilization, purification, and properties
24. 1 in rat liver
25. 1: A new metabolite
26. 1 exo- and endo-epoxides by rat and human glutathione S-transferases
27. 1 activated by cytochrome P450 1A enzymes
28. High efficiency transformation of intact yeast cells using single stranded nucleic acids as carriers
29. 1-8,9-epoxide conjugation activity in alpha class glutathione S-transferases through site-directed mutagenesis
30. 1-8, 9-epoxide conjugating activity in the non-human primate Macaca fascicularis liver
31. 1 by human, mouse and rat liver preparations: Effect on SCE in human mononuclear leucocytes
32. The interaction between microsomal epoxide hydrolase polymorphisms and cumulative cigarette smoking in different histological subtypes of lung cancer