Effective gene transfer into Trypanosoma brucei bloodstream forms by particle bombardment

Molecular and Biochemical Parasitology

Volumn 119, Issue 1, 2002, Pages 117-119

  Hara, Tatsuru ^a   Yasuda, Kouichi ^a   Fukuma, Toshihide ^a  

^a KURUME UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

Bloodstream forms; Gene transfer; Particle bombardment; Trypanosoma brucei

Indexed keywords

LUCIFERASE; PROTOZOAL DNA;

ARTICLE; BIOLISTIC TRANSFORMATION; DNA TRANSFECTION; ELECTROPORATION; ENZYME ACTIVITY; GENE TRANSFER; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; TECHNIQUE; TRYPANOSOMA BRUCEI;

ANIMALS; BIOLISTICS; LUCIFERASES; PLASMIDS; TRANSFECTION; TRYPANOSOMA BRUCEI BRUCEI;

PROTOZOA; TRYPANOSOMA; TRYPANOSOMA BRUCEI;

EID: 0036140150     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(01)00384-X     Document Type: Article

References (9)

1. Transfection of Leishmania and Trypanosoma brucei by electroporation
2. Transfection of the African and American trypanosomes
3. Optimization of biolistic transformation using the helium-driven PDS-1000/He system
4. Gene transfer into mammalian cells by particle bombardment
5. Autonomously replicating single-copy episomes in Trypanosoma brucei show unusual stability
6. Rapid uptake and phosphorylation of D-mannose, and limited D-mannose 6-phosophate isomerization in the glycolytic pathway of bloodstream forms of Trypanosoma brucei gambiense
7. Continuous cultivation of Trypanosoma brucei blood stream forms in a medium containing a low concentration of serum protein without feeder cell layers
8. Cultivation and in vitro cloning of procyclic culture forms of Trypansoma brucei in a semi-defined medium
9. Optimized use of the firefly luciferase assay as a reporter gene in mammalian cell lines