Identification of novel immunogenic proteins of Helicobacter pylori by proteome technology

Journal of Immunological Methods

Volumn 259, Issue 1-2, 2002, Pages 1-10

  Utt, M ^a   Nilsson, I ^a   Ljungh, A ^a   Wadstrom T ^a  

^a LUND UNIVERSITY   (Sweden)

Author keywords

Cell surface proteins; Helicobacter pylori; Heparin binding proteins; Immunoblot; Proteomics; Two dimensional electrophoresis

Indexed keywords

BACTERIAL PROTEIN; CELL SURFACE PROTEIN; GLYCINE; PEPTIDE; PROTEIN P25; PROTEIN P30; PROTEOME; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ARTICLE; BACTERIAL INFECTION; BACTERIUM; BIOTECHNOLOGY; CELL SURFACE; CONTROLLED STUDY; DIAGNOSTIC TEST; GRAM NEGATIVE INFECTION; HELICOBACTER PYLORI; IMMUNOBLOTTING; MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; SEROLOGY; SERUM; TWO DIMENSIONAL GEL ELECTROPHORESIS;

ANTIGENS, BACTERIAL; BACTERIAL OUTER MEMBRANE PROTEINS; CROSS REACTIONS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; HELICOBACTER INFECTIONS; HELICOBACTER PYLORI; HUMANS; IMMUNOBLOTTING; PROTEOME; SEROLOGIC TESTS;

BACTERIA (MICROORGANISMS); HELICOBACTER PYLORI;

EID: 0036133117     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(01)00476-8     Document Type: Article

References (29)

1. Use of immunoblot assay to define serum antibody patterns associated with Helicobacter pylori infection and with H. pylori-related ulcers
2. Serum antibody response to the superficial and released components of Helicobacter pylori
3. Helicobacter pylori and non-ulcer dyspepsia
4. Sulfated polysaccharide-directed recruitment of mammalian host proteins: A novel strategy in microbial pathogenesis
5. Multi-laboratory comparison of eight commercially available Helicobacter pylori serology kits
6. Two-dimensional map of Haemophilus influenzae following protein enrichment by heparin chromatography
7. Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography
8. Microbiological and serological diagnostic tests for Helicobacter pylori: An overview
9. Improved silver staining procedure for fast staining in PhastSystem Development Unit: I. Staining of sodium dodecyl sulfate gels
10. Glycosaminoglycan-protein interactions: Definition of consensus sites in glycosaminoglycan binding proteins
11. Comparative proteome analysis of Helicobacter pylori
12. Enrichment of human brain proteins by heparin chromatography
13. Identification of immunodominant antigens from Helicobacter pylori and evaluation of their reactivities with sera from patients with different gastroduodenal pathologies
14. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
15. Detection of antibodies to Helicobacter pylori cell surface antigens
16. Comparative analysis of structurally defined heparin binding sequences reveals a distinct spatial distribution of basic residues
17. Helicobacter pylori
18. Characterization of a Helicobacter pylori vaccine candidate by proteome techniques
19. Identification of potential diagnostic and vaccine candidates of Helicobacter pylori by two-dimensional gel-electrophoresis, sequence analysis, and serum profiling
20. Evaluation of eight enzyme immunoassays for detection of immunoglobulin G against Helicobacter pylori
21. Immunoblot assay for serodiagnosis of Helicobacter pylori infections
22. Two-dimensional electrophoretic and immunoblot analysis of cell surface proteins of spiral-shaped and coccoid forms of Helicobacter pylori
23. Solubilisation of proteins in two-dimensional electrophoresis
24. Optimal survival of Helicobacter pylori under various transport conditions
25. The complete genome sequence of the gastric pathogen Helicobacter pylori
26. Antibody to a Helicobacter pylori species specific antigen in patients with adenocarcinoma of the stomach
27. Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry
28. Search for putative virulence factors of Helicobacter pylori: The low-molecular-weight (33-35 K) antigen