The AFT024 stromal cell line supports long-term ex vivo maintenance of engrafting multipotent human hematopoietic progenitors

Leukemia

Volumn 16, Issue 3, 2002, Pages 352-361

  Nolta, J A ^a   Thiemann, F T ^a   Arakawa Hoyt, J ^a   Dao, M A ^a   Barsky, L W ^a   Moore, K A ^a   Lemischka, I R ^a   Crooks, G M ^a  

^a CHILDREN S HOSPITAL LOS ANGELES   (United States)

Author keywords

Ex vivo culture; Hematopoietic stem cells; Microenvironment; Xenotransplantation

Indexed keywords

CD19 ANTIGEN; CD33 ANTIGEN; CD34 ANTIGEN; CD38 ANTIGEN; CD4 ANTIGEN; CD45 ANTIGEN; CD8 ANTIGEN;

ANIMAL CELL; ANIMAL MODEL; ARTICLE; BONE MARROW; BONE MARROW CELL; CELL CULTURE; CELL DIFFERENTIATION; CELL GROWTH; CELL IMMORTALIZATION; CELL LINE; CELL LINEAGE; CELL SURVIVAL; COCULTURE; COLONY FORMING UNIT; CONTROLLED STUDY; FETUS; HEMATOPOIETIC STEM CELL; HEMATOPOIETIC STEM CELL TRANSPLANTATION; HUMAN; HUMAN CELL; IN VITRO STUDY; LYMPHOID CELL; MONOLAYER CULTURE; MOUSE; NONHUMAN; NUDE MOUSE; PHENOTYPE; PRIORITY JOURNAL; STROMA; STROMA CELL; TIME; UMBILICAL CORD BLOOD; XENOGRAFT;

ADP-RIBOSYL CYCLASE; ANIMALS; ANTIGENS, CD; ANTIGENS, CD38; ANTIGENS, DIFFERENTIATION; B-LYMPHOCYTES; BONE MARROW; BONE MARROW CELLS; BONE MARROW TRANSPLANTATION; CELLS, CULTURED; FETAL BLOOD; GRAFT SURVIVAL; HEMATOPOIESIS; HEMATOPOIETIC STEM CELL TRANSPLANTATION; HEMATOPOIETIC STEM CELLS; HUMANS; IMMUNOPHENOTYPING; MEMBRANE GLYCOPROTEINS; MICE; MICE, SCID; NAD+ NUCLEOSIDASE; STROMAL CELLS; T-LYMPHOCYTES; TRANSPLANTATION, HETEROLOGOUS;

EID: 0036119115     PISSN: 08876924     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.leu.2402371     Document Type: Article

References (42)

1. In vitro maintenance of highly purified, transplantable hematopoietic stem cells
2. The murine stromal cell line AFT024 acts specifically on human CD34+CD38- progenitors to maintain primitive function and immunophenotype in vitro
3. The type of stromal feeder used in limiting dilution assays influences frequency and maintenance assessment of human long-term culture initiating cells
4. The myeloid-lymphoid initiating cell (ML-IC) assay assesses the fate of multipotent human progenitors in vitro
5. Factor(s) secreted by AFT024 fetal liver cells following stimulation with human cytokines are important for human LTC-IC growth
6. A novel in vitro model of early human adult B lymphopoiesis that allows proliferation of pro-B cells and differentiation to mature B lymphocytes
7. Multi-lineage expansion potential of primitive hematopoietic progenitors. Superiority of umbilical cord blood compared to mobilized peripheral blood
8. Single adult human CD34(+)/Lin-/CD38(-) progenitors give rise to natural killer cells, B-lineage cells, dendritic cells, and myeloid cells
9. Isolation and characterization of human CD34(-)Lin(-) and CD34(+)Lin(-) hematopoietic stem cells using cell surface markers AC133 and CD7
10. During ontogeny primitive (CD34(+)CD38(-)) hematopoietic cells show altered expression of a subset of genes associated with early cytokine and differentiation responses of their adult counterparts
11. -) cells generated in a xenogenic coculture system are capable of both long-term hematopoiesis and multiple differentiation
12. Purification of primitive human hematopoietic cells capable of repopulating immune-deficient mice
13. -) human hematopoietic cells: Novel responses to Flt3-ligand and thrombopoietin
14. A functional comparison of CD34+ CD38- cells in cord blood and bone marrow
15. A murine stromal cell line allows the proliferation of very primitive human CD34++/CD38- progenitor cells in long-term cultures and semisolid assays
16. Sequential generations of hematopoietic colonies derived from single nonlineage-committed CD34+CD38- progenitor cells
17. Extrathymic human T lymphocyte development and regulation in immune deficient mice
18. IL-7 enhances the responsiveness of human T cells that develop in the bone marrow of athymic mice
19. Sustained human hematopoiesis in immunodeficient mice by cotransplantation of marrow stroma expressing human interleukin-3: Analysis of gene transduction of long-lived progenitors
20. Use of the bnx/hu xenograft model of human hematopoiesis to optimize methods for retroviral-mediated stem cell transduction (review)
21. Immunodeficient mice as models of human hematopoietic stem cell engraftment
22. The nonobese diabetic scid mouse: Model for spontaneous thymomagenesis associated with immunodeficiency
23. CD34: To select or not to select? That is the question
24. Engraftment and retroviral marking of CD34+ and CD34+CD38- human hematopoietic progenitors assessed in immune-deficient mice
25. Transduction of pluripotent human hematopoietic stem cells demonstrated by clonal analysis after engraftment in immune-deficient mice
26. Long-term cytokine production from engineered primary human stromal cells influences human hematopoiesis in an in vivo xenograft model
27. The number and generative capacity of human B lymphocyte progenitors, measured in vitro and in vivo, is higher in umbilical cord blood than in adult or pediatric bone marrow
28. A newly discovered class of human hematopoietic cells with SCID-repopulating activity
29. Rapid differentiation of a rare subset of adult human lin(-)CD34(-)CD38(-) cells stimulated by multiple growth factors in vitro
30. Clonal diversity of primitive human hematopoietic progenitors following retroviral marking and long-term engraftment in immune-deficient mice
31. Expansion of human cord blood CD34(+)CD38(-) cells in ex vivo culture during retroviral transduction without a corresponding increase in SCID repopulating cell (SRC) frequency: Dissociation of SRC phenotype and function
32. Quantitative analysis reveals expansion of human hematopoietic repopulating cells after short-term ex vivo culture
33. Hematopoietic activity of a stromal cell transmembrane protein containing epidermal growth factor-like repeat motifs
34. Pref-1, a protein containing EGF-like repeats, inhibits adipocyte differentiation
35. dlk, a putative mammalian homeotic gene differentially expressed in small cell lung carcinoma and neuroendocrine tumor cell line
36. Flt3 ligand induces proliferation of quiescent human bone marrow CD34+CD38- cells and maintains progenitor cells in vitro
37. Extended long-term culture reveals a highly quiescent and primitive human hematopoietic progenitor population
38. In vitro identification of single CD34+CD38- cells with both lymphoid and myeloid potential
39. Kinetics of engraftment of CD34(-) and CD34(+) cells from mobilized blood differs from that of CD34(-) and CD34(+) cells from bone marrow
40. Ex vivo generation of CD34(+) cells from CD34(-) hematopoietic cells
41. Reversible expression of CD34 by murine hematopoietic stem cells
42. Reversibility of CD34 expression on human stem cells that retain the capacity for secondary reconstitution