Functional analysis of the Escherichia coli molybdopterin cofactor biosynthesis protein MoeA by site-directed mutagenesis

Biological Chemistry

Volumn 383, Issue 2, 2002, Pages 319-323

  Sandu, Cristinel ^a   Brandsch, Roderich ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

Author keywords

Mo MGD (MoCo); Mo MPT; MoeA; MPT

Indexed keywords

ALANINE; AMINO ACID; ASPARTIC ACID; BACTERIAL PROTEIN; GLYCINE; MOLYBDIC ACID; MOLYBDOPTERIN; MUTANT PROTEIN; NITRATE REDUCTASE; PROLINE;

AMINO ACID SUBSTITUTION; ARTICLE; BIOSYNTHESIS; CELL TRANSFORMATION; CONTROLLED STUDY; CORRELATION FUNCTION; CRYSTAL STRUCTURE; ENZYME ACTIVITY; ESCHERICHIA COLI; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IN VITRO STUDY; IN VIVO STUDY; NEUROSPORA CRASSA; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEPHOSPHORYLATION; PROTEIN FUNCTION; SITE DIRECTED MUTAGENESIS; WILD TYPE;

BLOTTING, WESTERN; COENZYMES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENES, BACTERIAL; METALLOPROTEINS; MUTAGENESIS, SITE-DIRECTED; NITRATE REDUCTASE; NITRATE REDUCTASES; PTERIDINES; SULFURTRANSFERASES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEUROSPORA; NEUROSPORA CRASSA;

EID: 0036118794     PISSN: 14316730     EISSN: None     Source Type: Journal    
DOI: 10.1515/BC.2002.034     Document Type: Article

References (23)

1. Characterization of molybdenum cofactor from Escherichia coli
2. The nucleotide sequence and protein-coding capability of the transposable element IS 5
3. Involvement of a b-type cytochrome in the assimilatory nitrate reductase of Neurospora crassa
4. Phenotypic restoration by molybdate of nitrate reductase activity in chID mutants of Escherichia coli
5. Physiological and genetic analyses leading to identification of a biochemical role for the moeA (molybdate metabolism) gene product in Escherichia coli
6. Transcriptional regulation of the moe (molybdate metabolism) operon of Escherichia coli
7. Effect of molybdate and tungstate on the biosynthesis of CO dehydrogenase and the molybdopterin cytosine-dinucleotide-type of molybdenum cofactor in Hydrogenophaga pseudoflava
8. Molybdenum cofactor biosynthesis in Escherichia coli. Requirement of the chIB gene product for the formation of molybdopterin guanine dinucleotide
9. Activity of the molybdopterin-containing xanthine dehydrogenase of Rhodobacter capsulatus can be restored by high molybdenum concentrations in a moeA mutant defective in molybdenum cofactor biosynthesis
10. In vitro incorporation of nascent molybdenum cofactor into human sulfite oxidase
11. Characterization of Escherichia coli MoeB and its involvement in the activation of MPT synthase for the biosynthesis of the molybdenum cofactor
12. Crystal structure of the gephyrin-related molybdenum cofactor biosynthesis protein MogA from Escherichia coli
13. A new set of useful cloning and expression vectors derived from pBlueScript
14. Isolation of protein FA, a product of the mob locus required for molybdenum cofactor biosynthesis in Escherichia coli
15. The pterin molybdenum cofactors
16. Nitrate reductase activity and heterocyst supression on nitrate in Anabena sp. strain PCC 7120 require moeA
17. Crystal structure of DMSO reductase: Redox-linked changes in molybdopterin coordination
18. The nucleotide sequence of IS5 from Escherichia coli
19. Molecular characterization of the gene cluster coxMSL encoding the molybdenum-containing carbon monoxide dehydrogenase of Oligotropha carboxidovorans
20. The neurotransmitter receptor-anchoring protein gephyrin reconstitutes molybdenum cofactor biosynthesis in bacteria, plants, and mammalian cells
21. Transductuin of nitrate reductase loci of Escherichia coli by phages P1 and λ
22. The crystal structure of Escherichia coli MoeA and its relationship to the multifunctional protein gephyrin