Use of antisperm antibodies in differential display Western blotting to identify sperm proteins important in fertility

Human Reproduction

Volumn 17, Issue 4, 2002, Pages 984-989

  Chiu, W W C ^a   Chamley, L W C ^a  

^a UNIVERSITY OF AUCKLAND   (New Zealand)

Author keywords

Antisperm antibodies; IgG; Infertility; Spermatozoa; Vasectomy

Indexed keywords

IMMUNOGLOBULIN G; PROTEIN; SPERM ANTIBODY; SPERM ANTIGEN;

ANTIBODY DETECTION; ANTIGEN SPECIFICITY; ARTICLE; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST; DIFFERENTIAL DISPLAY; HUMAN; MALE; MALE FERTILITY; MALE INFERTILITY; MALE STERILIZATION REVERSAL; PROGNOSIS; PROTEIN ANALYSIS; PROTEIN BINDING; QUANTITATIVE ANALYSIS; SENSITIVITY AND SPECIFICITY; SERUM; SPERMATOZOON MOTILITY; VASECTOMY; WESTERN BLOTTING;

EID: 0036115822     PISSN: 02681161     EISSN: None     Source Type: Journal    
DOI: 10.1093/humrep/17.4.984     Document Type: Article

References (37)

1. Immunology of semen
2. Interaction of immunoglobulin fragments with the mammalian sperm acrosome
3. Identification of human sperm surface glycoproteins by sperm membrane-specific autoiantibodies
4. Sperm-associated and circulating IgA and IgG classes of antibodies recognise different antigens on the human sperm plasma membrane
5. Conception rates in couples where autoimmunity to sperm in detected
6. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
7. Antisperm antibodies: A critical evaluation and clinical guidelines
8. Identification of an oolemmal IgG Fc receptor: Its role in promoting binding of antibody-labelled human sperm to zona-free hamster eggs
9. Monoclonal antibodies identify Fc gamma receptors on unfertilized human oocytes but not spermatozoa
10. Improved chemiluminescent detection of alkaline phosphatase
11. Frequency and predictive value of antisperm antibodies among infertile couples
12. Corticosteroid treatment does not improve the results of intrauterine insemination in male subfertility caused by antisperm antibodies
13. Risk factors for antisperm antibodies in infertile men
14. Solubilization of membranes by detergents
15. The significance of antisperm antibodies: Measurement and management
16. Quantitative determination of IgG and IgA on sperm from infertile patients with and without antisperm antibodies
17. Identification of IgG and Fc-binding proteins human seminal plasma and sperm
18. Blot-immunobinding test for the detection of anti-sperm antibodies
19. Special on sperm from autoimmune infertile men
20. Antisperm antibodies and fertility after vasovasostomy: A follow-up study of 216 men
21. Fecundity an infertility in the United States: Incidence and trends (editorial; comment)
22. Auto- and iso-antigens of human spermatozoa detected by immunoblotting with human sera after SDS-PAGE
23. Sperm processing in the female reproductive tract
24. The effects of sperm antibodies on fertility after vasectomy reversal
25. Characterization of sperm antigens reacting with human antisperm antibodies
26. Detection and quantitation of sperm-bound antibodies by flow cytometry of human semen
27. Non-immune IgC binding to the surface of spermatozoa by disulphide rearrangement
28. Reversal of vasectomy: The effects of sperm antibodies on subsequent fertility
29. Membrane cofactor protein (MCP, CD46) in seminal plasma and on spermatozoa in normal and 'sterile' subjects
30. Identification of human sperm antigens reacting with antisperm antibodies from sera and genital tract secretions
31. Presence of the complement-regulatory protein membrane cofactor protein (MCP, CD46) as a membrane-associated product in seminal plasma
32. Prevalence of antisperm antibodies by SpermMARtest in subjects undergoing a routine sperm analysis for intertility
33. Complement-binding proteins are strongly expressed by human preimplantation blastocysts and cumulus cells as well gametes
34. Inhibition of human spermatozoon-oocyte interaction in vitro by monoclonal antibodies to CD46 (membrane cofactor protein)
35. The epidemiology of infertility in Aberdeen
36. The biologic significance of white blood cells in semen