Interaction between lactate dehydrogenase and Tween 80 in aqueous solution

Pharmaceutical Research

Volumn 19, Issue 4, 2002, Pages 504-510

  Hillgren, Anna ^a   Evertsson, Hans ^a   Aldén, Maggie ^a  

^a UPPSALA UNIVERSITY   (Sweden)

Author keywords

Fluorescent probes; Protein denaturation; Protein surfactant interaction; Pulsed field gradient spin echo NMR

Indexed keywords

AMPHOPHILE; LACTATE DEHYDROGENASE; POLYSORBATE 80; PYRENE; SURFACTANT;

AQUEOUS SOLUTION; ARTICLE; DIFFERENTIAL SCANNING CALORIMETRY; DRUG FORMULATION; FLUORESCENCE; MICELLE; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DENATURATION; PROTEIN INTERACTION; PROTEIN PROTEIN INTERACTION; SPECTROFLUOROMETRY; VISCOSITY;

ANIMALS; CALORIMETRY, DIFFERENTIAL SCANNING; DRUG INTERACTIONS; L-LACTATE DEHYDROGENASE; PHARMACEUTICAL SOLUTIONS; POLYSORBATES; PYRENES; RABBITS; SPECTROMETRY, FLUORESCENCE; VISCOSITY; WATER;

EID: 0036104695     PISSN: 07248741     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1015156031381     Document Type: Article

References (22)

1. The mechanism of cryoprotection of proteins by solutes
2. Surface-induced denaturation of proteins during freezing and its inhibition by surfactants
3. Effect of Tween 20 on freeze-thawing- and agitation-induced aggregation of recombinant human factor XIII
4. Instability, stablilization, and formulation of liquid protein pharmaceuticals
5. Protein-solvent interactions in pharmaceutical formulations
6. Strucutre of lactate dehydrogenase at 2.8 Å resolution
7. Freeze-thaw studies of a model protein, lactate dehydrogenase, in the presence of cryoprotectants
8. Spectroscopic probe analysis of protein-surfactant interactions: The BSA/SDS system
9. Interactions of globular proteins with surfactants studied with fluorescence probe methods
10. Environmental effects on vibronic band intensities in pyrene monomer fluorescence and their applications in studies on micellar systems
11. Intramolecular eximer formation with diar-kylalkanes as a microfluidity probe for sodium dodecyl sulphate micelles
12. Fourier transform pulsed-gradient spin-echo studies of molecular diffusion
13. Use of the stimulated echo in NMR diffusion studies
14. Global least-squares analysis of large, correlated spectral data sets: Application to component resolved FT-PGSE NMR spectroscopy
15. Temperature effects on the interactions between EHEC and SDS in dilute aqueous solutions. Steady-state fluorescence quenching and equilibrium dialysis investigations
16. CMC of polysorbates
17. Microviscosity of aqueous surfactant micesses: Effect of various parameters
18. Microviscosity in EHEC/SDS-clusters formed in dilute aqueous solutions as determined with fluorescence probe techniques
19. Mechanisms of phase behavior and protein partitioning in detergent/polymer aqueous two-phase systems for purification of integral membrane proteins
20. Tween protects recombinant human growth hormone against agitation-induced damage via hydrophobic interactions