The application of single-strand conformation polymorphism (SSCP) technique for the analysis of molecular heterogeneity of grapevine virus A

Vitis

Volumn 41, Issue 2, 2002, Pages 77-82

  Goszczynski, D E ^a   Jooste, A E C ^a  

^a PLANT PROTECTION RESEARCH INSTITUTE   (South Africa)

Author keywords

Cloning and sequencing; Isolates of grapevine virus A; RT PCR; SSCP

Indexed keywords

DNA FRAGMENT; RESTRICTION ENDONUCLEASE;

3' UNTRANSLATED REGION; CANADA; GENETIC VARIABILITY; GRAPEVINE VIRUS A; MOLECULAR SIZE; OPEN READING FRAME; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SINGLE STRAND CONFORMATION POLYMORPHISM; SWITZERLAND;

NICOTIANA BENTHAMIANA;

EID: 0036067686     PISSN: 00427500     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Polymorphism of the 5′ terminal region of Citrus tristeza virus (CTV) RNA: Incidence of three sequence types in isolates of different origin and pathogenicity
2. Production, characterization and use of monoclonal antibodies to grapevine virus A
3. Filamentous viruses of grapevine: Putative trichoviruses and capilloviruses
4. Excess PCR primers may dramatically affect SSCP efficiency
5. An immunocapture PCR assay adopted to the detection and analysis of the molecular variability of apple chlorotic leaf spot virus
6. Use of immunocapture-polymerase chain reaction for the detection of grapevine virus A in Kober stem grooving-infected grapevines
7. New primers for the molecular identification and detection of grapevine virus A (GVA)
8. On the possible relationship between Kober stem grooving and grapevine virus A
9. Western blots reveal that grapevine virus A and grapevine virus B are serologically related
10. Etiological studies and diagnosis of grapevine leafroll disease improved by monoclonal antibodies
11. Characterization of closterovirus-like particles associated with grapevine leafroll disease
12. Single-strand conformation polymorphism analysis of apple stem grooving capillovirus sequence variants
13. Vitivirus, a new genus of plant viruses
14. Rugose wood of grapevines
15. Detection of grapevine closterovirus A in infected grapevine tissue by reverse transcription-polymerase chain reaction
16. Grapevine virus A: Nucleotide sequence, genome organization, and relationship in the Trichovirus genus
17. Mechanical sap transmission of a closterovirus from in vitro shoot tip cultures of a leafroll-affected grapevine to Nicotiana benthamiana
18. Detection of two strains of grapevine virus A
19. Separation and interference of strains from a Citrus tristeza virus isolate evidenced by biological activity and double-stranded RNA (dsRNA) analysis
20. Detection of polymorphism of human DNA by gel electrophoresis as single-strand conformation polymorphisms
21. Differentiation of Citrus tristeza closterovirus (CTV) isolates by single-strand conformation polymorphism analysis of the coat protein gene
22. Analysis of double-stranded RNA for plant virus diagnosis