Optimisation of one-tube PCR-ELISA to detect femtogram amounts of genomic DNA

Journal of Microbiological Methods

Volumn 51, Issue 2, 2002, Pages 163-170

  Wilson, T ^a,c   Carson, J ^a   Bowman, J ^b  

^a UNIVERSITY OF TASMANIA   (Australia)

^b UNIVERSITY OF TASMANIA   (Australia)

^c DEPARTMENT OF PRIMARY INDUSTRIES   (Australia)

Author keywords

96 well; Hybridization; NucleoLink; PCR ELISA

Indexed keywords

4 NITROPHENYLPHOSPHATASE; ALKALINE PHOSPHATASE; BACTERIAL DNA; BIOTIN; STREPTAVIDIN;

AEROMONAS SALMONICIDA; ARTICLE; BACTERIAL GENOME; COLORIMETRY; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FLEXIBACTER MARITIMUS; GENE AMPLIFICATION; HYBRIDIZATION; LACTOCOCCUS GARVIEAE; NONHUMAN; PATHOGENESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SIGNAL DETECTION; SOLID; TENACIBACULUM MARITIMUM; YERSINIA RUCKERI;

ANIMALS; DNA, BACTERIAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; FISHES; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

AEROMONAS; AEROMONAS SALMONICIDA; BACTERIA (MICROORGANISMS); FLEXIBACTER; LACTOCOCCUS GARVIEAE; TENACIBACULUM; TENACIBACULUM MARITIMUM; YERSINIA; YERSINIA RUCKERI;

EID: 0036064960     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(02)00055-6     Document Type: Article

References (16)

1. Development of molecular probes for use in bacterial disease diagnosis and health monitoring of farmed and wild finfish in Australia
2. Miniaturized tests for computer-assisted identification of motile Aeromonas species with an improved probability matrix
3. Post-PCR sterilisation: A method to control carryover contamination for the polymerase chain reaction
4. PCR-ELISAs for the detection of Campylobacter jejuni and Campylobacter coli in poultry samples
5. Quantitative detection of meat spoilage bacteria by using the polymerase chain reaction (PCR) and an enzyme linked immunosorbent assay (ELISA)
6. Evaluation of a polymerase chain reaction-based assay for the detection of Aeromonas salmonicida ss salmonicida in Atlantic salmon Salmo salar
7. Avoiding false positives with PCR
8. A procedure for the isolation of deoxyribonucleic acid from micro-organisms
9. Quantification of picogram levels of specific DNA immobilized in microtiter wells
10. Detection of immobilized amplicons by ELISA-like techniques
11. Amplification product inactivation methods
12. Covalent immobilization of DNA onto polystyrene microwells: The molecules are only bound at the 5′ end
13. Combined polymerase chain reaction hybridization microplate assay used to detect bovine leukemia virus and Salmonella
14. Preventing false positives: Quantitative evaluation of three protocols for inactivation of polymerase chain reaction amplification products
15. Chemiluminescent and colorimetric enzymatic assays for the detection of PCR-amplified Salmonella sp. products in microplates
16. Simple colourimetric microtitre plate hybridization assay for detection of amplified mycobacterium leprae DNA