An evaluation of long-term preservation methods for brown bear (Ursus arctos) faecal DNA samples

Conservation Genetics

Volumn 3, Issue 4, 2002, Pages 435-440

  Murphy, Melanie A ^a   Waits, Lisette P ^a   Kendall, Katherine C ^b   Wasser, Samuel K ^c   Higbee, Jerry A ^d   Bogden, Robert ^d  

^a UNIVERSITY OF IDAHO   (United States)

^b U S GEOLOGICAL SURVEY   (United States)

^c UNIVERSITY OF WASHINGTON   (United States)

^d Amplicon Express   (United States)

Author keywords

DNA extraction; DNA preservation; Faecal DNA; Noninvasive genetic sampling; PCR; Ursus arctos

Indexed keywords

ASSAYS; BIOLOGICAL MATERIALS PRESERVATION; CONSERVATION; DNA; ETHANOL; HORMONES; SILICA; THERMAL EFFECTS;

ANIMAL CONSERVATION;

GENETIC ENGINEERING;

BEAR; DNA; FECES; METHODOLOGY; PRESERVATION;

ANIMALIA; URSUS ARCTOS;

EID: 0036032486     PISSN: 15660621     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1020503330767     Document Type: Article

References (11)

1. Frantzen MAJ, Silk JB, Ferguson JWH, Wayne RK, Kohn MH (1998) Empirical evaluation of preservation methods for fecal DNA. Molecular Ecology, 7, 1423-1428.
2. Gerloff U, Schlötterer C, Rassmann K et al. (1995) Amplification of hypervariable simple sequence repeats (microsatellites) from excremental DNA of wild living bonobos (Pan paniscus). Molecular Ecology, 4, 515-518.
3. Goossens B, Chikhi L, Utami SS, Ruiter Jd, Bruford MW (2000) A multi-samples, multi-extracts approach for microsatellite analysis of faecal samples in an arboreal ape. Conservation Genetics, 1, 157-162.
4. Kohn M, Wayne RK (1997) Facts from feces revisited. Trends in Ecology and Evolution, 12, 223-227.
5. Kohn MH, York EC, Kamradt DA et al. (1999) Estimating population size by genotyping faeces. Proceedings of the Royal Society of London, 266, 1-7.
6. Murphy MA, Waits LP, Kendall KC (2000) Quantitative evaluation of fecal drying methods for brown bear DNA analysis. Wildlife Society Bulletin, 28, 951-957.
7. Paetkau D, Calvert A, Stirling J, Strobeck C (1995) Microsatellite analysis of population structure in Canadian polar bears. Molecular Ecology, 4, 347-354.
8. Taberlet P, Griffin S, Goossens B et al. (1996) Reliable genotyping of samples with very low DNA quantities using PCR. Nucleic Acids Research, 24, 3189-3194.
9. Taberlet P, Waits LP, Luikart G (1999) Noninvasive genetic sampling: Look before you leap. Trends in Ecology and Evolution, 14, 323-327.
10. Wasser SK, Houston CS, Koehiler GM, Cadd GG, Fain SR (1997) Techniques for applications of faecal DNA methods to field studies of Ursids. Molecular Ecology, 6, 1091-1097.
11. Woods JG, Paetkau D, Lewis D, McLellan BN (1999) Genetic tagging of free-ranging black and brown bears. Wildlife Society Bulletin, 27, 616-627.