TaqMan junction probes and the reverse transcriptase polymerase chain reaction: Detection of alveolar rhabdomyosarcoma, synovial sarcoma, and desmoplastic small round cell tumor

Annals of Clinical and Laboratory Science

Volumn 32, Issue 3, 2002, Pages 219-224

  Cummings, Thomas J ^a   Brown, Nicholas M ^a   Stenzel, Timothy T ^a  

^a DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

Chromosomal translocations, chimeric gene fusion products; RT PCR; TaqMan junction probes

Indexed keywords

TAQ POLYMERASE; TUMOR ANTIGEN;

ARTICLE; CANCER CELL CULTURE; CHROMOSOME TRANSLOCATION; CORRELATION ANALYSIS; DESMOPLASTIC SMALL ROUND CELL TUMOR; GEL ELECTROPHORESIS; GENE AMPLIFICATION; GENE FUSION; GENE PROBE; GENETIC VARIABILITY; INTERMETHOD COMPARISON; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RHABDOMYOSARCOMA; SYNOVIAL SARCOMA; CELL CULTURE; COMPUTER SYSTEM; CONNECTIVE TISSUE TUMOR; HUMAN; MOLECULAR PROBE; SMALL CELL CARCINOMA;

CARCINOMA, SMALL CELL; COMPUTER SYSTEMS; HUMAN; MOLECULAR PROBES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RHABDOMYOSARCOMA, ALVEOLAR; SARCOMA, SYNOVIAL; TAQ POLYMERASE; TUMOR CELLS, CULTURED; HUMANS;

EID: 0036022321     PISSN: 00917370     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (33)

1. Fusion of a forkhead domain gene to PAX3 in the solid tumor alveolar rhabdomyosarcoma
2. Rearrangement of the PAX3 paired box gene in the paediatric solid tumour alveolar rhabdomyosarcoma
3. Fusion of PAX3 to a member of the forkhead family of transcription factors in human alveolar rhabdomyosarcoma
4. Fusion of PAX7 to FKHR by the variant t(1;13)(p36;q14) translocation in alveolar rhabdomyosarcoma
5. SYT-SSX gene fusion as a determinant of morphology and prognosis in synovial sarcoma
6. Fusion of SYT to two genes, SSX1 and SSX2, encoding proteins with homology to the Kruppel-associated box in human synovial sarcoma
7. Molecular diagnosis of synovial sarcoma and characterization of a variant SYT-SSX2 fusion transcript
8. Strong association of SYT-SSX fusion type and morphologic epithelial differentiation in synovial sarcoma
9. The t(X;18)(p11.2;q11.2) translocation found in human synovial sarcomas involves two distinct loci on the X chromosome
10. Identification of novel genes, SYT and SSX, involved in the t(X;18)(p11.2;q11.2) translocation found in human synovial sarcoma
11. Identification of two alternative fusion genes, SYT-SSX1 and SYT-SSX2, in t(X;18) (p11.2;q 11.2)-positive synovial sarcomas
12. Translocation (11;22)(p13;q12): Primary change in intraabdominal desmoplastic small round cell tumor
13. Detection of chimeric transcripts in desmoplastic small round cell tumor and related developmental tumors by reverse transcriptase polymerase chain reaction. A specific diagnostic assay
14. Intra-abdominal desmoplastic small round-cell tumor. Report of 19 cases of a distinctive type of high-grade polyphenotypic malignancy affecting young individuals
15. Detection of the EWS/WT1 gene fusion by reverse transcriptase-polymerase chain reaction in the diagnosis of intra-abdominal desmoplastic small round cell tumor
16. Molecular variants of the EWS-WT1 gene fusion in desmoplastic small round cell tumor
17. Real time quantitative PCR
18. Continuous fluorescence monitoring of rapid cycle DNA amplification
19. Molecular assays for chromosomal translocations in the diagnosis of pediatric soft tissue sarcomas
20. A multiplex realtime PCR assay for the detection of gene fusions observed in solid tumors
21. Monitoring of BCR-ABL expression using real-time RT-PCR in CML after bone marrow or peripheral blood stem cell transplantation
22. Detection of minimal residual disease in patients with AML1/ETO-associated acute myeloid leukemia using a novel quantitative reverse transcription polymerase chain reaction assay
23. The molecular biology of chronic myeloid leukaemia
24. A second Ewing's sarcoma translocation, t(21;22), fuses the EWS gene to another ETS-family transcription factor, ERG
25. Combinatorial generation of variable fusion proteins in the Ewing family of tumors
26. EWS-FLI1 fusion transcript structure is an independent determinant of prognosis in Ewing's sarcoma
27. Does expression of different EWS chimeric transcripts define clinically distinct risk groups of Ewing tumor patients?
28. Molecular biology of Ewing's sarcoma/primitive neuroectodermal tumor family
29. Differential transactivation by alternative EWS-FLI1 fusion proteins correlates with clinical heterogeneity in Ewing's sarcoma
30. Molecular mechanisms underlying human synovial sarcoma development
31. Antigenicity of fusion proteins from sarcoma-associated chromosomal translocations
32. Fluorescence in situ hybridization: Molecular probes for diagnosis of pediatric neoplastic diseases