Spatially modulated illumination microscopy allows axial distance resolution in the nanometer range

Applied Optics

Volumn 41, Issue 1, 2002, Pages 80-87

  Albrecht, Benno ^a   Virgilio Failla, Antonio ^a   Schweitzer, Andreas ^a   Cremer, Christoph ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CHARGE COUPLED DEVICES; ENERGY TRANSFER; FLUORESCENCE; OPTICAL MICROSCOPY; PHOTONS; SUPRAMOLECULAR CHEMISTRY;

FAR-FIELD LASER FLUORESCENCE MICROSCOPY;

NANOSTRUCTURED MATERIALS;

ALGORITHM; ARTICLE; FLUORESCENCE MICROSCOPY; LASER; NANOTECHNOLOGY;

ALGORITHMS; LASERS; MICROSCOPY, FLUORESCENCE; NANOTECHNOLOGY;

EID: 0036017335     PISSN: 1559128X     EISSN: 21553165     Source Type: Journal    
DOI: 10.1364/AO.41.000080     Document Type: Article

References (36)

1. T. Cremer and C. Cremer, “Chromosome territories and the functional nuclear architecture, ” Nat. Rev. 2, 292-301 (2001).
2. H. Bornfleth, K. Satzler, R. Eils, and C. Cremer, “High-precision distance measurements and volume-conserving segmentation of objects near and below the resolution limit in three-dimensional confocal fluorescence microscopy, ” J. Mi-crosc. 189, 118-136 (1998).
3. A. Esa, P. Edelmann, G. Kreth, L. Trakhtenbrot, N. Amariglio, G. Rechavi, M. Hausmann, and C. Cremer, “Threedimensional spectral precision distance microscopy of chromatin nanostructures after triple-colour DNA labelling: a study of the BCR region on chromosome 22 and the Philadelphia chromosome, ” J. Microsc. 199, 96-105 (2000).
4. M. Van Oijen, J. Kohler, J. Schmidt, M. Muller, and G. J. Brakenhoff, “3-dimensional super-resolution by spectrally selective imaging, ” Chem. Phys. Lett. 292, 183-187 (1998).
5. T. D. Lacoste, X. Michalet, F. Pinaud, D. S. Chemla, A. P. Alivisatos, and S. Weiss, “Ultrahigh-resolution multicolor colocalization of single fluorescent probes, ” Proc. Natl. Acad. Sci. USA 97, 9461-9466 (2000).
6. P. Edelmann, A. Esa, M. Hausmann, and C. Cremer, “Confocal laser-scanning microscopy: in situ determination of the confocal point-spread function and the chromatic shifts in intact cell nuclei, ” Optik 110, 194-198 (1999).
7. E. Schrock, S. du Manoir, T. Veldman, B. Schoell, J. Wienberg, M. A. Ferguson-Smith, Y. Ning, D. H. Ledbetter, I. Bar-Am, D. Soenksen, Y. Garini, and T. Reid, “Multicolor spectral karyotyping of human chromosomes, ” Science 273, 494-497 (1996).
8. M. R. Speicher, S. G. Ballard, and D. C. Ward, “Karyotyping human chromosomes by combinatorial multi-fluor FISH, ” Nat. Genet. 12, 368-375 (1996).
9. J. R. Lakowicz, H. Szmaczinski, and K. Nowaczyk, “Fluorescence lifetime imaging, ” Proc. Natl. Acad. Sci. USA 89, 1271-1275 (1992).
10. Schoenle, M. Glatz, and S. W. Hell, “Four-dimensional multiphoton microscopy with time-correlated single-photon counting, ” Appl. Opt. 39, 6306-6311 (2000).
11. D. M. J. Lilley and T. J. Wilson, “Fluorescence resonance energy transfer as a structural tool for nucleic acids, ” Curr. Opin. Chem. Biol. 4, 507-517 (2000).
12. P. Edelmann and C. Cremer, “Improvement of confocal spectral precision distance microscopy (SPDM), ” in Optical Diagnostics of Living Cells III, D. L. Farkas and R. C. Leif, eds., Proc. SPIE 3921, 313-320 (2000).
13. H. Bornfleth, P. Edelmann, D. Zink, T. Cremer, and C. Cremer, “Quantitative motion analysis of subchromosomal foci in living cells using four-dimensional microscopy, ” Biophys. J. 77, 2871-2886 (1999).
14. C. Cremer and T. Cremer, “Considerations on a laserscanning-microscope with high resolution and depth of field, ” Microsc. Acta 81, 31-44 (1978).
15. S. W. Hell, S. Lindek, C. Cremer, and E. H. K. Stelzer, “Measurement of 4pi-confocal point spread function proves 75 nm axial resolution, ” Appl. Phys. Lett. 64, 1335-1337 (1994).
16. P. E. Hanninen, S. W. Hell, J. Salo, E. Soini, and C. Cremer, “Two-photon excitation 4Pi confocal microscope: enhanced axial resolution microscope for biological research, ” Appl. Phys. Lett. 66, 1698-1700 (1995).
17. M. Schrader, K. Bahlmann, G. Giese, and S. W. Hell, “4Pi-confocal imaging in fixed biological specimen, ” Biophys. J. 75, 1659-1668 (1998).
18. S. W. Hell and J. Wichmann, “Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy, ” Opt. Lett. 19, 780-782 (1994).
19. T. A. Klar, S. Jakobs, M. Dyba, A. Egner, and S. W. Hell, “Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission, ” Proc. Natl. Acad. Sci. USA 97, 8206-8210 (2000).
20. M. Schmidt, M. Nagorny, and S. W. Hell, “Subresolution axial measurements in far-field fluorescence microscopy with precision of 1 nanometer, ” Rev. Sci. Instrum. 71, 2742-2745 (2000).
21. B. Schneider, I. Upmann, I. Kirsten, J. Bradl, M. Hausmann, and C. Cremer, “A dual-laser, spatially modulated illumination fluorescence microscope, ” Microsc. Anal. 57, 5-7 (1999).
22. B. Schneider, B. Albrecht, P. Jaeckle, D. Neofotistos, S. Soding, T. Jager, and C. Cremer, “Nanolocalization measurements in spatially modulated illumination microscopy using two coherent illumination beams, ” in Optical Diagnostics of Living Cells III, D. L. Farkas and R. C. Leif, eds., Proc. SPIE 3921, 321-330 (2000).
23. B. Bailey, D. Farkas, D. Taylor, and F. Lanni, “Enhancement of axial resolution in fluorescence microscopy by standing-wave excitation, ” Nature 366, 44-48 (1993).
24. M. G. L. Gustafsson, D. A. Agard, and J. W. Sedat, “Sevenfold improvement of axial resolution in 3D wide-field microscopy using two objective-lenses, ” in Three-Dimensional Microscopy: Image Acquisition and Processing II, T. Wilson and C. J. Cogswell, eds., Proc. SPIE 2412, 147-156 (1995).
25. W. H. Press, S. A. Teukolsky, W. T. Vetterling, and B. P. Flannery, Numerical Recipes in C, 2nd ed. (University of Cambridge, Cambridge, UK, 1992).
26. Khoral Research Inc., 6200 Uptown Blvd. N.E., Suite 200, Albuquerque, N. Mex. 87110-4142, http://www.khoral.com.
27. B. Albrecht, A. V. Failla, R. Heintzmann, and C. Cremer, “Spatially modulated illumination microscopy: online visualization of intensity distribution and prediction of nanometer precision of axial distance measurements by computer simulations, ” J. Biomed. Opt. (to be published).
28. R. Heintzmann, G. Kreth, and C. Cremer, “Reconstruction of axial tomographic high resolution data from confocal fluorescence microscopy: a method for improving 3D FISH images, ” Anal. Cell. Path. 20, 7-15 (2000).
29. M. Hausmann, C. Cremer, J. Bradl, and B. Schneider, “Wellenfeldmikroskop, Wellenfeldmikroskopieverfahren, auch zur DNA-Sequenzierung, und Kalibrierverfahren fur die Wellenfeldmikroskopie” (Wavefield microscopy, wavefield microscopy procedures, also for DNA sequencing), German patent application DE 19830569A1 (7 July 1998).
30. C. Cremer, P. Edelmann, H. Bornfleth, G. Kreth, H. Muench, H. Luz, and M. Hausmann, “Principles of spectral precision distance confocal microscopy of molecular nuclear structure, ” in Handbook of Computer Vision and Applications, B. Jähne, H. Haussecker, and P. Geissler, eds. (Academic, San Diego, Calif., 1999), Vol. 3.
31. G. Marriott, R. M. Clegg, D. J. Arndt-Jovin, and T. M. Jovin, “Time-resolved imaging microscopy. Phosphorescence and delayed fluorescence imaging, ” Biophys. J. 60, 1374-1387 (1991).
32. T. Cremer, A. Kurz, R. Zirbel, S. Dietzel, B. Rinke, E. Schrock, M. R. Speicher, U. Mathieu, A. Jauch, P. Emmerich, H. Scher-than, T. Ried, C. Cremer, and P. Lichter, “Role of chromosome territories in the functional compartmentalization of the cell nucleus, ” Cold Spring Harb. Symp. Quant. Biol. 58, 777-792 (1993).
33. A. I. Lamond and W. C. Earnshaw, “Structure and function in the nucleus, ” Science 280, 547-553 (1998).
34. T. Cremer, G. Kreth, H. Koester, R. H. A. Fink, R. Heintzmann, I. Solovei, D. Zink, and C. Cremer, “Chromosome territories, interchromatin domain compartment and nuclear matrix: an integrated view of the functional nuclear architecture, ” Crit. Rev. Eukaryotic Gene Expression 12, 179 -212 (2000).
35. B. Albrecht, A. V. Failla, A. Schweitzer, and C. Cremer, “Spatially modulated illumination microscopy, ” G. I. T. Imag. Mi-crosc. 2, 40-42 (2001).
36. V. Failla, A. Cavallo, and C. Cremer are preparing a manuscript to be called “Subwavelength size determination with SMI virtual microscopy.”