Random mutagenesis targeted to the psbAII gene of Synechocystis sp. PCC 6803 to identify functionally important residues in the D1 protein of the photosystem II reaction center

Plant and Cell Physiology

Volumn 43, Issue 5, 2002, Pages 540-548

  Yamasato, Akihiro ^a,b   Kamada, Tomoe ^a,c   Satoh, Kimiyuki ^a,d  

^a OKAYAMA UNIVERSITY   (Japan)

^b HOKKAIDO UNIVERSITY   (Japan)

^c NATIONAL INSTITUTE FOR BASIC BIOLOGY   (Japan)

^d ARIZONA STATE UNIVERSITY   (United States)

Author keywords

D1 protein; Photosystem II; psbA gene; Random mutagenesis; Synechocystis sp. PCC 6803

Indexed keywords

PROKARYOTA; SYNECHOCYSTIS; SYNECHOCYSTIS SP.; SYNECHOCYSTIS SP. PCC 6803;

CHLOROPHYLL; PHOTOSYSTEM II, PSBA SUBUNIT;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; CYANOBACTERIUM; GENETICS; LIGHT; METABOLISM; MOLECULAR GENETICS; MUTAGENESIS; PHOTOSYNTHESIS; PHOTOSYSTEM II; POINT MUTATION; RADIATION EXPOSURE; THYLAKOID;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; CHLOROPHYLL; CYANOBACTERIA; LIGHT; MOLECULAR SEQUENCE DATA; MUTAGENESIS; PHOTOSYNTHETIC REACTION CENTER COMPLEX PROTEINS; PHOTOSYSTEM II PROTEIN COMPLEX; POINT MUTATION; THYLAKOIDS;

EID: 0036016854     PISSN: 00320781     EISSN: None     Source Type: Journal    
DOI: 10.1093/pcp/pcf066     Document Type: Article

References (26)

1. Photosynthetic mutants of the cyanobacteria Synechocystis sp. strains PCC 6714 and PCC 6803: Sodium p-hydroxymercuribenzoate as a selective agent
2. Randomization of genes by PCR mutagenesis
3. Amino acid residues that influence the binding of manganese or calcium to photosystem II. 2. The carboxy-terminal domain of the D1 polypeptide
4. Amino acid residues that influence the binding of manganese or calcium to photosystem II. 1. The lumenal interhelical domains of the D1 polypeptide
5. Mutagenesis of His D1-198 and His D2-197 in Synechocystis PCC 6803: Effects on the primary donor of photosystem II
6. Z and the manganese cluster in the water oxidizing complex of photosystem II
7. + in photosystem II is tyrosine- 161 of the D1 polypeptide
8. Site-directed mutations at D1-His198 and D2-His197 of photosystem II in Synechocystis PCC 6803: Sites of primary charge separation and cation and triplet stabilization
9. Random chemical mutagenesis of a specific psbDI region coding for a lumenal loop of the D2 protein of photosystem II in Synechocystis sp. PCC 6803
10. Targeted random mutagenesis to identify functionally important residues in the D2 protein of photosystem II in Synechocystis sp. strain PCC 6803
11. Selection for mutants in energetic pathways: Enrichment procedures
12. Characterization of a heterotrophic mutant of Synechocystis sp. PCC 6803 created by random mutagenesis targeted to the psbAII gene
13. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
14. Isolation of a photosystem II reaction center consisting of D-1 and D-2 polypeptides and cytochrome b-559
15. The herbicide-resistant species of the cyanobacterial D1 protein obtained by thorough and random in vitro mutagenesis
16. Photo-tolerant mutants of Synechocystis sp. PCC 6803 obtained by in vitro random mutagenesis of psbA2
17. Aspartate 170 of the photosystem II reaction center polypeptide D1 is involved in the assembly of the oxygen-evolving manganese cluster
18. Isolation and purification of cyanobacteria
19. Molecular cloning and characterization of the ctpA gene encoding a carboxyl-terminal processing protease
20. Biochemical and spectroscopic characterization of a new oxygen-evolving photosystem II core complex from the cyanobacterium Synechocystis PCC 6803
21. The topology of the plastoquinone and herbicide binding peptides of photosystem II in the thylakoid membrane
22. Engineering of the protein environment around the redox-active TyrZ in photosystem II. The role of F186 and P162 in the D1 protein of Synechocystis 6803
23. Construction of specific mutations in PSII photosynthetic reaction center by genetic engineering
24. Selection of PSII-deficient mutants of Synechocystis sp. PCC 6803 created by targeted in vitro random mutagenesis of psbAII gene based on nitrofurantoin resistance
25. The establishment of conditions to efficiently screen photosynthesis-deficient mutants of Synechocystis sp. PCC 6803 by nitrofurantoin treatment
26. Crystal structure of photosystem II from Synechococcus elongatus at 3.8 Å resolution