Antibodies obtained by xenotransplantation of organ-cultured median eminence specifically recognize hypothalamic tanycytes

Cell and Tissue Research

Volumn 308, Issue 2, 2002, Pages 241-253

  Blázquez, Juan ^a   Guerra, Montserrat ^b   Pastor, Francisco ^a   Peruzzo, Bruno ^b   Amat, Pedro ^a   Rodríguez, Esteban ^b  

^a UNIVERSITY OF SALAMANCA   (Spain)

^b UNIVERSIDAD AUSTRAL DE CHILE   (Chile)

Author keywords

Bovine; Median eminence; Organ culture; Rat (Sprague Dawley); Specific marker; Tanycytes; Xenotransplantation

Indexed keywords

ANTIBODY; ANTIGEN; ANTISERUM; GONADORELIN; PROTEIN; PROTEIN P60; PROTEIN P85; UNCLASSIFIED DRUG; NERVE PROTEIN; TISSUE EXTRACT;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CATTLE; CELL LABELING; CENTRAL NERVOUS SYSTEM; CONTROLLED STUDY; GONADOTROPIN RELEASE; HYPOTHALAMUS; IMMUNOCHEMISTRY; IMMUNOHISTOCHEMISTRY; IMMUNOREACTIVITY; MEDIAN EMINENCE; NONHUMAN; ORGAN CULTURE; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; TANYCYTE; XENOTRANSPLANTATION; ANIMAL; CELL VACUOLE; CYTOLOGY; ELECTRON MICROSCOPY; FEMALE; IMMUNOBLOTTING; IMMUNOLOGY; MALE; METABOLISM; ORGAN CULTURE TECHNIQUE; SPRAGUE DAWLEY RAT; TRANSPLANTATION; ULTRASTRUCTURE; XENOGRAFT;

ANIMAL; CATTLE; FEMALE; HYPOTHALAMUS; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; MALE; MEDIAN EMINENCE; MICROSCOPY, ELECTRON; NERVE TISSUE PROTEINS; ORGAN CULTURE; RATS; RATS, SPRAGUE-DAWLEY; SUPPORT, NON-U.S. GOV'T; TISSUE EXTRACTS; TRANSPLANTATION, HETEROLOGOUS; VACUOLES; ANIMALS; ORGAN CULTURE TECHNIQUES;

ANIMALIA; BOS TAURUS; BOVINAE;

EID: 0036015704     PISSN: 0302766X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00441-002-0528-9     Document Type: Article

References (52)

1. Morphological aspects of the hypothalamic-hypophyseal system. VI. The tanycytes: Their relation to the sexual differentiation of the hypothalamus. An enzyme-histochemical study
2. Tanycytes and their relation to the hypophyseal gonadotropic function
3. Lateral evaginations from the third ventricle into the rat mediobasal hypothalamus: An amplification of the ventricular route
4. A system of intraependymal cisternae along the margins of the median eminence in the rat: Structure, three-dimensional arrangement and ontogeny
5. Transmission and scanning electron-microscopic observations on tanycytes in the mediobasal hypothalamus and the median eminence of adrenalectomized rats
6. Estradiol and progesterone regulate the expression of insulin-like growth factor-I receptor and insulin-like growth factor binding protein-2 in the hypothalamus of adult female rats
7. Transforming growth factor-α
8. Kainate glutamate receptors (GluR5-7) in the rat arcuate nucleus: Relationship to tanycytes, astrocytes, neurons and gonadal steroid receptors
9. Gonadal hormone regulation of insulin-like growth factor-I-like immunoreactivity in hypothalamic astroglia of developing and adult rats
10. Distribution of plectin, an intermediate filament-associated protein, in the adult rat central nervous system
11. The hypothalamic arcuate nucleus-median eminence complex: Immunohistochemistry of transmitters, peptides and DARPP-32 with special reference to coexistence in dopamine neurons
12. Identification of kainate-preferring glutamate receptor subunit GluR7 mRNA and protein in the rat median eminence
13. DARPP-32 and CREB are present in type 2 iodothyronine deiodinase-producing tanycytes: Implications for the regulation of type 2 deiodinase activity
14. Endocrine-dependent accumulation of IGF-I by hypothalamic glia
15. Tanycytes: Morphology and function: A review
16. Localization of insulin-like growth factor I (IGF-I)-like immunoreactivity in the developing and adult rat brain
17. Role of astroglia in estrogen regulation of synaptic plasticity and brain repair
18. Soluble factors guide gonadotropin-releasing hormone axonal targeting to the median eminence
19. Possible site of action of dopamine in the hypothalamic pituitary control
20. DARPP-32 as a marker for D-1 dopaminoceptive cells in the rat brain: Prenatal development and presence in glial elements (tanycytes) in the basal hypothalamus
21. Die faserglia des selachiergehirns
22. Dynamic changes in LHRH neurovascular terminals with various endocrine conditions in adults
23. Ependymoneuronal specialisation between LHRH fibers and cells of the cerebroventricular system
24. Cleavage of structural proteins during the assembly of the head bacteriophage T4
25. Estrogen receptor immunoreactive glia, endothelia, and ependyma in guinea pig preoptic area and median eminence: Electron microscopy
26. Organization of the neuroglia in the midsagittal plane of the central nervous system: A speculative report
27. Ovarian steroids differentially regulate the expression of PRL-R in neuroendocrine dopaminergic neuron populations: A double label confocal micoscopic study
28. Hypothalamic astrocytes respond to transforming growth factor-α with the secretion of neuroactive substances that stimulate the release of luteinizing hormone-releasing hormone
29. LHRF, LHRH, GnRH: What controls the secretion of this hormone?
30. Fine structure of the median eminence of the rat
31. Immunohistochemical localization in the rat brain of an epitope corresponding to the fibroblast growth factor receptor-1
32. Glial cells and neuroendocrine function
33. DARPP-32, a dopamine- and cyclic AMP-regulated phosphoprotein in tanycytes of the mediobasal hypothalamus: Distribution and relation to dopamine and luteinizing hormone-releasing hormone neurons and other glial elements
34. Neurotransmitters, neuropeptides and binding sites in the rat mediobasal hypothalamus: Effects of monosodium glutamate (MSG) lesions
35. Type 1 astrocytes influence luteinizing hormone-releasing hormone release from the hypothalamic cell line GT1-1: Is transforming growth factor-β the principle involved?
36. Epidermal growth factor tyrosine kinase receptors and the neuroendocrine control of mammalian puberty
37. Involvement of transforming growth factor-α in the release of luteinizing-hormone releasing hormone from the developing female hypothalamus
38. Neurotrophic factors and female sexual development
39. The transforming growth factor-α gene family is involved in the neuroendocrine control of mammalian puberty
40. A second look at the barriers of the medial basal hypothalamus
41. Definitive evidence for the existence of morphological plasticity in the external zone of the median eminence during the rat estrous cycle: Implication of neuroglioendothelial interactions in gonadotropin-releasing hormone release
42. Glial cells positive for glial fibrillary acidic protein in the neurohypophysis of the Djungarian hamster (Phodopus sungorus)
43. The cerebrospinal fluid as a pathway in neuroendocrine integration
44. Cellular organization of the lateral and postinfundibular regions of the median eminence in the rat
45. Evidence for the participation of the CSF and periventricular structures in certain neuroendocrine mechanisms
46. Involvement of tanycytes in the control of gonadotropin secretion
47. Ultrastructural changes in the median eminence of the rat following deafferentation of the basal hypothalamus
48. The unlabeled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes
49. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications
50. SuperSignal CL HRP: A new enhanced chemiluminescent substrate for the development of the horseradish peroxide label in Western blotting applications
51. Tanycytes and pituicytes: Morphological and functional aspects of neuroglial interaction