Characterization of the proteolytic enzymes in the midgut of the European Cockchafer, Melolontha melolontha (Coleoptera: Scarabaeidae)

Insect Biochemistry and Molecular Biology

Volumn 32, Issue 7, 2002, Pages 803-814

  Wagner, Wolfgang ^a   Möhrlen, Frank ^a   Schnetter, Wolfgang ^a  

^a Institute of Zoology   (Germany)

Author keywords

Activity blot; Bacillus thuringiensis; Insecticidal crystal proteins; Melolontha melolontha; Midgut proteinases; Zymogram

Indexed keywords

SERINE PROTEINASE; AMINOPEPTIDASE; ASTACIN; METALLOPROTEINASE; PROTEINASE;

ANIMAL; ARTICLE; BEETLE; CELL MEMBRANE; DIGESTIVE SYSTEM; ENZYME SPECIFICITY; ENZYMOLOGY; METABOLISM; MOLECULAR WEIGHT; BRUSH BORDER VESICLE; COLEOPTERA; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; FLUOROMETRY; MELOLONTHA MELOLONTHA; MIDGUT; NONHUMAN; PHOTOMETRY; ZYMOGRAPHY;

ANIMALS; BEETLES; CELL MEMBRANE; DIGESTIVE SYSTEM; MOLECULAR WEIGHT; SERINE ENDOPEPTIDASES; SUBSTRATE SPECIFICITY; ANIMAL;

BACILLUS THURINGIENSIS; BACILLUS THURINGIENSIS SEROVAR JAPONENSIS; COLEOPTERA; CRUSTACEA; INSECTA; MELOLONTHA MELOLONTHA; SCARABAEIDAE;

EID: 0036015624     PISSN: 09651748     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0965-1748(01)00167-9     Document Type: Article

References (49)

1. New chloride-activated aminopeptidase from human erythrocytes
2. Resolution and partial characterization of the digestive proteinases from larvae of the black carpet beetle
3. T cell-depleted bone marrow transplantation and delayed T cell add-back to control acute GVHD and conserve a graft-versus-leukemia effect
4. Recent advances in matrix metalloproteinase inhibitor research
5. Blood digestion in the mosquito. Anopheles stephensi Liston (Diptera: Culicidae): Partial characterization and post-feeding activity of midgut aminopeptidases
6. Purification and characterization of a digestive Cathepsin D proteinase isolated from Tribolium castaneum larvae (Herbst)
7. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
8. Partial purification and characterization of the major midgut protenases of grass grub larvae (Costelytra zealandica, Coleoptera: Scarabaeidae)
9. A gene family in Drosophila melanogaster coding for trypsin-like enzymes
10. Differential chemical reactivities of zinc in horse liver alcohol dehydrogenase
11. Differences in the midgut proteolytic activity of two Heliothis virescens strains, one susceptible and one resistant to Bacillus thuringiensis toxins
12. Substrate-gel electrophoresis for composition and molecular weight of proteinases or proteinaceous proteinase inhibitors
13. Characterization of major midgut proteinase cDNAs from Helicoverpa armigera larvae and changes in gene expression in response to four proteinase inhibitors in the diet
14. Electrophoretic analysis of plasminogen activators in polyacrylamide gels containing sodium dodecyl sulfate and copolymerized substrates
15. Ectoenzymes of the kidney microvillar membrane. Differential solubilization by detergents can predict a glycosyl-phosphatidylinositol membrane anchor
16. Characterization of larvicidal toxin protein from Bacillus thuringiensis serovar japonensis strain Buibui specific for scarabaeid beetles
17. The partial purification and characterisation of serine protease activity in midgut of larval Helicoverpa armigera
18. Insect pathogenic soil fungi from Melolontha melolontha control sites in the canton Thurgau
19. Digestion of [delta]-endotoxin by gut proteases may explain reduced sensitivity of advanced instar larvae of Spodoptera littoralis to CryIC
20. Metal binding to angiotensin converting enzyme: Implications for the metal binding site
21. Heterologously overexpressed, affinity-purified human meprin alpha is functionally active and cleaves components of the basement membrane in vitro
22. Glutathione-degrading enzymes of microvillus membranes
23. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
24. Characterisation of multiple trypsins from the midgut of Locusta migratoria
25. Soluble and membrane-bound forms of trypsin-like enzymes in Musca domestica larval midguts
26. Isolation and characterization of a new trypsin-like enzyme from Tenebrio molitor L. larvae
27. The aminopeptidase from hog intestinal brush border
28. Structured and catalytic properties of peptidase N from Escherichia coli K-12
29. Peptide hydroxamic acids inhibit skin collagenase
30. A unique isolate of Bacillus thuringiensis serovar japonensis with a high larvicidal activity specific for scarabaeid beetles
31. Review: Protease interactions with Bacillus thuringiensis insecticidal toxins
32. Luminal proteinases from Plodia interpunctella and the hydrolysis of Bacillus thuringiensis CryIA(c) protoxin
33. Insect resistance to Bacillus thuringiensis toxins
34. Susceptibility of Melolontha melolontha to Heterorhabditis bacteriophora, H. megidis and Steinernema glaseri
35. Sequence of three cDNAs encoding an alkaline midgut trypsin from Manduca sexta
36. Evolutionary families of peptidases
37. Insect proteinases
38. Control of the cockchafer Melolontha melolontha in the Kraichgau with NeemAzal-T/S
39. Fluorescent oligopeptide substrates for kinetic characterization of the specificity of Astacus protease
40. Astacus protease, a zinc metalloenzyme
41. Astacin
42. Gypsy moth midgut proteinases: Purification and characterization of luminal trypsin elastase and the brush border membrane leucine aminopeptidase
43. Why is the scarab specific Bacillus thuringiensis ssp japonensis strain Buibui inefficient against Melolontha spec
44. Isolation and partial characterization of a major gut proteinase from larval Acanthoscelides obtectus Say (Coleoptera: Bruchidae)
45. Preparation and partial characterization of amino acid transporting brush border membrane vesicles from larval midgut of the cabbage butterfly (Pieris brassicae)
46. Thiol containing compounds and amino acid hydroxamates as reversible synthetic inhibitors of Astacus protease
47. Phosphinic peptides, the first potent inhibitors of astacin, behave as extremely slow-binding inhibitors