Molecular analysis of the cytosolic Dictyostelium γ-tubulin complex

European Journal of Cell Biology

Volumn 81, Issue 4, 2002, Pages 175-184

  Daunderer, Christine ^a   Gräf, Ralph ^a  

^a UNIVERSITY OF MUNICH   (Germany)

Author keywords

Centrosome; Dictyostelium; MTOC; Spc97; Spc98; tubulin

Indexed keywords

CYTOPLASM PROTEIN; GAMMA TUBULIN; SODIUM CHLORIDE; HYBRID PROTEIN; MICROTUBULE ASSOCIATED PROTEIN; PROTOZOAL PROTEIN; TUBGCP2 PROTEIN, HUMAN; TUBGCP3 PROTEIN, HUMAN; TUBULIN;

ARTICLE; CELL CYCLE; CELL LINE; CELL MUTANT; CENTROSOME; COMPLEX FORMATION; CONTROLLED STUDY; CYTOSOL; DICTYOSTELIUM; MICROTUBULE ASSEMBLY; MOLECULAR BIOLOGY; MOLECULAR CLONING; MOLECULAR SIZE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; SEQUENCE HOMOLOGY; AMINO ACID SEQUENCE; ANIMAL; CHEMISTRY; CLASSIFICATION; CYTOLOGY; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MICROTUBULE; MOLECULAR GENETICS; MUTATION; PHYLOGENY; PHYSIOLOGY; REPORTER GENE; SEQUENCE ALIGNMENT;

DICTYOSTELIUM; PROTOZOA;

AMINO ACID SEQUENCE; ANIMALS; CELL CYCLE; CLONING, MOLECULAR; DICTYOSTELIUM; GENES, REPORTER; HUMANS; MICROTUBULE-ASSOCIATED PROTEINS; MICROTUBULES; MOLECULAR SEQUENCE DATA; MUTATION; PHYLOGENY; PROTOZOAN PROTEINS; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT; TUBULIN;

EID: 0036011160     PISSN: 01719335     EISSN: None     Source Type: Journal    
DOI: 10.1078/0171-9335-00241     Document Type: Article

References (67)

1. Isolation of Dictyostelium discoideum cytokinesis mutants by restriction enzyme-mediated integration of the blasticidin S resistance marker
2. Characterization of γ-tubulin complexes in Aspergillus nidulans and detection of putative γ-tubulin interacting proteins
3. Electron microscopic mapping of monoclonal antibodies on the tail region of Dictyostelium myosin
4. Dictyostelium discoideum: A promising centrosome model system
5. Dictyostelium centrin-related protein (DdCrp), the most divergent member of the centrin family, possesses only two EF-hands and dissociates from the centrosome during mitosis
6. Polar organization of γ-tubulin in acentriolar mitotic spindles of Drosophila melanogaster cells
7. Protein complexes containing γ-tubulin are present in mammalian brain microtubule protein preparations
8. Pericentrin and γ-tubulin form a protein complex and are organized into a novel lattice at the centrosome
9. Protofilaments and rings, two conformations of the tubulin family conserved from bacterial FtsZ to α/β and γ-tubulin
10. Dictyostelium γ-tubulin: Molecular characterization and ultrastructural localization
11. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product
12. Human 76p: A new member of the γ-tubulin-associated protein family
13. Centrosome assembly in vitro: Role of γ-tubulin recruitment in Xenopus sperm aster formation
14. PHYLIP - Phylogeny interference package (Version 3.2)
15. The spindle pole body component Spc98p interacts with the γ-tubulin-like Tub4p of Saccharomyces cerevisiae at the sites of microtubule attachment
16. Comparative structural, molecular and functional aspects of the Dictyostelium discoideum centrosome
17. Cell cycle-dependent localization of monoclonal antibodies raised against isolated Dictyostelium centrosomes
18. Dictyostelium DdCP224 is a microtubule-associated protein and a permanent centrosomal resident involved in centrosome duplication
19. Isolation of nucleation-competent centrosomes from Dictyostelium discoideum
20. γ-Tubulin complexes and their role in microtubule nucleation
21. Characterization and reconstitution of Drosophila γ-tubulin ring complex subunits
22. The use of cell division cycle mutants to investigate the control of microtubule distribution in the fission yeast Schizosaccharomyces pombe
23. The fission yeast γ-tubulin is essential for mitosis and is localized at microtubule organizing centers
24. A novel structural component of the Dictyostelium centrosome
25. The Dictyostelium genome project: An invitation to species hopping
26. The centrosome and cellular organization
27. The spindle pole body component Spc97p interacts with the γ-tubulin of Saccharomyces cerevisiae and functions in microtubule organization and spindle pole body duplication
28. Spc98p and Spc97p of the yeast γ-tubulin complex mediate binding to the spindle pole body via their interaction with Spc110p
29. Receptors determine the cellular localization of a γ-tubulin complex and thereby the site of microtubule formation
30. Microtubule-nucleating activity of centrosomes in Chinese hamster ovary cells is independent of the centriole cycle but coupled to the mitotic cycle
31. Electroblotting of multiple gels: A simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose
32. A single γ-tubulin gene and mRNA, but two γ-tubulin polypeptides differing by their binding to the spindle pole organizing centres
33. Expression of amino- and carboxyl-terminal γ- and α-tubulin mutants in cultured epithelial cells
34. Cell biological, molecular genetic, and biochemical methods used to examine Dictyostelium
35. In vitro assembly of multiprotein complexes containing a, b, and γ-tubulin, heat shock protein HSP70, and elongation factor 1α
36. Analysis of Tub4p, a yeast γ-tubulin-like protein: Implications for microtubule-organizing center function
37. Xgrip109: A γ-tubulin-associated protein with an essential role in γ-tubulin ring complex (γTuRC) assembly and centrosome function
38. Polarity and nucleation of microtubules in polarized epithelial cells
39. Chaperonin-mediated folding of vertebrate actin-related protein and γ-tubulin
40. Spindle and kinetochore morphology of Dictyostelium discoideum
41. Microtubule nucleation by γ-tubulin-containing rings in the centrosome
42. γ-tubulin in mammalian cells: The centrosomal and the cytosolic forms
43. The mammalian γ-tubulin complex contains homologues of the yeast spindle pole body components Spc97p and Spc98p
44. Molecular biology in Dictyostelium: Tools and applications
45. Identification of γ-tubulin, a new member of the tubulin superfamily encoded by mipA gene of Aspergillus nidulans
46. Characterization of two related Drosophila γ-tubulin complexes that differ in their ability to nucleate microtubules
47. Dictyostelium MTOC: Structure and linkage to the nucleus
48. The evolution of the mitotic apparatus: An attempt at comparative ultrastructural cytology in dividing plant cells
49. Drosophila g-tubulin is part of a complex containing two previously identified centrosomal MAPs
50. Fine structure of an organelle associated with the nucleus and cytoplasmic microtubules in the cellular slime mould Polysphondylium violaceum
51. Indirect immunofluorescence of microtubules in Dictyostelium discoideum. A study with polyclonal and monoclonal antibodies to tubulins
52. Components of the yeast spindle and spindle pole body
53. γ-Tubulin is a highly conserved component of the centrosome
54. In vitro reconstitution of centrosome assembly and function: The central role of γ-tubulin
55. Characterization of the human homologue of the yeast Spc98p and its association with γ-tubulin
56. Centrosome positioning and directionality of cell movements
57. Unusual centrosome cycle in Dictyostelium: Correlation of dynamic behavior and structural changes
58. Identification of a complex between centrin and heat shock proteins in CSF-arrested Xenopus oocytes and dissociation of the complex following oocyte activation
59. Identification of intrinsic dimer and overexpressed monomeric forms of γ-tubulin in Sf9 cells infected with baculovirus containing the Chlamydomonas g-tubulin sequence
60. Centrosomes isolated from Spisula solidissima oocytes contain rings and an unusual stoichiometric ratio of α/β tubulin
61. Wild-type strains of Dictyostelium discoideum can be transformed using a novel selection cassette driven by the promoter of the ribosomal V18 gene
62. Cell-density-dependent repression of discoidin in Dictyostelium discoideum
63. Spindle Pole Body of Saccharomyces cerevisiae: A Model for Genetic Analysis of the Centrosome Cycle
64. Review: An overview of the Saccharomyces cerevisiae microtubule and microfilament cytoskeleton
65. The role of Xgrip210 in γ-tubulin ring complex assembly and centrosome recruitment
66. γ-tubulin is present in Drosophila melanogaster and Homo sapiens and is associated with the centrosome
67. Nucleation of microtubule assembly by a γ-tubulin-containing ring complex