Transgenic expression in arabidopsis of a polyprotein construct leading to production of two different antimicrobial proteins

Plant Physiology

Volumn 128, Issue 4, 2002, Pages 1346-1358

  François, Isabelle E J A ^a   De Bolle, Miguel F C ^a   Dwyer, Geoff ^a   Goderis, Inge J W M ^a   Woutors, Piet F J ^a   Verhaert, Peter D ^a   Proost, Paul ^a   Schaaper, Wim M M ^a   Cammue, Bruno P A ^a   Broekaert, Willem F ^a  

^a UNIVERSITY OF LEUVEN   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

GENETIC ENGINEERING; PROTEINS; SEED;

ANTIFUNGAL ACTIVITY;

PLANTS (BOTANY);

ARABIDOPSIS; DAHLIA; DAHLIA MERCKII; EMBRYOPHYTA; EUKARYOTA; IMPATIENS; IMPATIENS BALSAMINA; RAPHANUS SATIVUS;

ANTIFUNGAL AGENT; ANTIINFECTIVE AGENT; ANTIMICROBIAL CATIONIC PEPTIDE; DEFENSIN; DM AMP1 PROTEIN, DAHLIA MERCKII; DM-AMP1 PROTEIN, DAHLIA MERCKII; GLUCOSE 6 PHOSPHATE DEHYDROGENASE; HYBRID PROTEIN; PLANT EXTRACT; POLYPROTEIN; RSAFP2 PROTEIN, RAPHANUS SATIVUS; VEGETABLE PROTEIN;

AMINO ACID SEQUENCE; ARABIDOPSIS; ARTICLE; CHEMISTRY; DRUG EFFECT; FUSARIUM; GENE EXPRESSION REGULATION; GENETICS; IMPATIENS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; PLASMID; SEQUENCE ALIGNMENT; TRANSGENIC PLANT;

AMINO ACID SEQUENCE; ANTI-INFECTIVE AGENTS; ANTIFUNGAL AGENTS; ANTIMICROBIAL CATIONIC PEPTIDES; ARABIDOPSIS; DEFENSINS; FUSARIUM; GENE EXPRESSION REGULATION, PLANT; GLUCOSEPHOSPHATE DEHYDROGENASE; IMPATIENS; MOLECULAR SEQUENCE DATA; PLANT EXTRACTS; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED; PLASMIDS; POLYPROTEINS; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT;

EID: 0036010657     PISSN: 00320889     EISSN: None     Source Type: Journal    
DOI: 10.1104/pp.010794     Document Type: Article

References (37)

1. Nucleotide sequence of the T-DNA region from Agrobacterium tumefaciens octopine Ti plasmid pTi15955
2. In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants
3. New plant binary vectors with selectable markers located proximal to the left T-DNA border
4. Expression of multiple eukaryotic genes from a single promoter in Nicotiana
5. Structure and transcription of the nopaline synthase gene region of T-DNA
6. Phytodetoxification of hazardous organomercurials by genetically engineered plants
7. A rapid and sensitive method for the quantification of microgram quantities utilizing the principle of protein-dye binding
8. An automated quantitative assay for fungal growth inhibition
9. Antimicrobial peptides from plants
10. Plant defensins: Novel antimicrobial peptides as components of the host defense system
11. A viral cleavage site cassette: Identification of amino acid sequences required for tobacco etch virus polyprotein processing
12. Containment of herbicide resistance through genetic engineering of the chloroplast genome
13. An aspartic endoproteinase present in seeds cleaves Arabidopsis 2S albumins in vitro
14. High-throughput RNA extraction from plant samples based on homogenisation by reciprocal shaking in the presence of a mixture of sand and glass beads
15. Identification of the motifs within the tobacco mosaic virus 5′-leader responsible for enhancing translation
16. A set of modular plant transformation vectors allowing flexible insertion of up to six expression units
17. Self-processing 2A-polyproteins: A system for coordinate expression of multiple proteins in transgenic plants
18. Internal initiation of mRNA translation in eukaryotes
19. Duplication of CaMV35S promoter sequences creates a strong enhancer for plant genes
20. Development in matrix-assisted laser desorption/ionization peptide mass spectrometry
21. In vitro characterization of a cassette to accumulate multiple proteins through synthesis of a self-processing polypeptide
22. Position effects and epigenetic silencing of plant genes
23. Enhanced expression of an antimicrobial peptide sarcotoxin IA by GUS fusion in transgenic tobacco plants
24. Isolation and characterization of plant defensins from seeds of Asteraceae, Fabaceae, Hippocastanaceae and Saxifragaceae
25. Pathogen-induced systemic activation of a plant defensin gene in Arabidopsis follows a salicylic-independent pathway
26. Construction of chimeric molecules by a two-step recombinant PCR method
27. Enzymes of the glycolytic and pentose phosphate pathways in proplastids from the developing endosperm of Ricinus communis L
28. Metabolic engineering of Arabidopsis and Brassica for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer production
29. A novel family of small cysteine-rich antimicrobial peptides from seeds of Impatiens balsamina is derived from a single precursor protein
30. Small cysteine-rich antifungal proteins from radisch: Their role in host defense
31. Analysis of two novel classes of plant antifungal proteins from radish (Raphanus sativus L.) seeds
32. Enhanced transgenic plant resistance to nematodes by dual proteinase inhibitor constructs
33. Virus and fungal resistance: From laboratory to field
34. The endoplasmic reticulum-gateway of the secretory pathway
35. A cassette containing the bar gene of Streptomyces hygroscopicus: A selectable marker for plant transformation
36. Plant antifungal proteins
37. Enhanced protection against fungal attack by constitutive co-expression of chitinase and glucanase genes in transgenic tobacco