Defining a 5′ splice site by functional selection in the presence and absence of U1 snRNA 5′ end

RNA

Volumn 8, Issue 2, 2002, Pages 166-179

  Lund, Mette ^a   Kjems, Jørgen ^a  

^a AARHUS UNIVERSITY   (Denmark)

Author keywords

5 splice site; In vitro selection; RNA splicing; U1 snRNA; U1 snRNP

Indexed keywords

MESSENGER RNA PRECURSOR; SMALL NUCLEAR RIBONUCLEOPROTEIN; SMALL NUCLEAR RNA; CAPPED RNA; U1 SMALL NUCLEAR RNA; U5 SMALL NUCLEAR RNA; U6 SMALL NUCLEAR RNA;

ARTICLE; BASE PAIRING; COMPLEX FORMATION; GENE MUTATION; HELA CELL; HUMAN; HUMAN CELL; INTRON; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RNA SEQUENCE; RNA SPLICING; SPLICEOSOME; 3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; CHEMISTRY; DNA TEMPLATE; GENE LIBRARY; GENETICS; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PROMOTER REGION;

METAZOA;

3' UNTRANSLATED REGIONS; 5' UNTRANSLATED REGIONS; BASE SEQUENCE; GENE LIBRARY; HELA CELLS; HUMANS; INTRONS; MOLECULAR SEQUENCE DATA; PROMOTER REGIONS (GENETICS); RNA CAPS; RNA SPLICING; RNA, SMALL NUCLEAR; TEMPLATES, GENETIC;

EID: 0036009859     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: 10.1017/S1355838202010786     Document Type: Article

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6. Allele-specific genetic interactions between Prp8 and RNA active site residues suggest a function for Prp8 at the catalytic core of the spliceosome
7. Mutations in the conserved loop of human U5 snRNA generate use of novel cryptic 5′ splice sites in vivo
8. Complementation by SR proteins of pre-mRNA splicing reactions depleted of U1 snRNP
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14. Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns
15. U1-specific protein C needed for efficient complex formation of U1 snRNP with a 5′ splice site
16. The 100-kda U5 snRNP protein (hPrp28p) contacts the 5′ splice site through its ATPase site
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19. Site-specific crosslinks of yeast U6 snRNA to the pre-mRNA near the 5′ splice site
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27. Site-specific modification of pre-mRNA: The 2′-hydroxyl groups at the splice sites
28. A catalogue of splice junction sequences
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31. Mutations in yeast U5 snRNA alter the specificity of 5′ splice-site cleavage
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33. Splicing of messenger RNA precursors
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35. The C-terminal region of hPrp8 interacts with the conserved GU dinucleotide at the 5′ splice site
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37. Sequence logos: A new way to display consensus sequences
38. A U1 snRNA:pre-mRNA base pairing interaction is required early in yeast spliceosome assembly but does not uniquely define the 5′ cleavage site
39. Exon mutations uncouple 5′ splice site selection from U1 snRNA pairing
40. Probing of the spliceosome with site-specifically derivatized 5′ splice site RNA oligonucleotides
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42. Functional interactions of Prp8 with both splice sites at the spliceosomal catalytic center
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44. The U5 and U6 small nuclear RNAs as active site components of the spliceosome
45. An RNA switch at the 5′ splice site requires ATP and the DEAD box protein Prp28p
46. PRP28, a "DEAD-box" protein, is required for the first step of mRNA splicing in vitro
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48. SR proteins can compensate for the loss of U1 snRNP functions in vitro
49. Extensive interactions of PRP8 protein with the 5′ and 3′ splice sites during splicing suggest a role in stabilization of exon alignment by U5 snRNA
50. Interactions of small nuclear RNA's with precursor messenger RNA during in vitro splicing
51. In vitro reconstitution of mammalian U1 snRNPs active in splicing: The U1-C protein enhances the formation of early (E) spliceosomal complexes
52. Specific interactions between proteins implicated in splice site selection and regulated alternative splicing
53. Site-specific cross-linking of mammalian U5 snRNP to the 5′ splice site before the first step of pre-mRNA splicing
54. Identification of eight proteins that crosslink to pre-mRNA in the yeast commitment complex
55. A biochemical function for the Sm complex
56. A compensatory base change in U1 snRNA suppresses a 5′ splice site mutation