Preparation of a conjugate of 2′,5′-triadenylate 5′-triphosphate and biotinylated firefly luciferase and its use for sensitive bioluminescent enzyme immunoassay

Bioconjugate Chemistry

Volumn 13, Issue 2, 2002, Pages 303-308

  Seto, Yoshiaki ^a,b   Abe, Katsushi ^a   Itoh, Masao ^a   Sawai, Hiroaki ^b  

^a EIKEN CHEMICAL CO LTD   (Japan)

^b GUNMA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYMES; IMMUNOLOGY; PROBES;

AEROBIC CONDITION; AEROBIC OXIDATIONS; COMPLEX FORMATIONS; DIMERIZATIONS; DIRECT ANALYSIS; ENZYME IMMUNOASSAY; FIREFLY LUCIFERASE; MALEIMIDES; STREPTAVIDIN; THIOL GROUPS;

BIOLUMINESCENCE;

LUCIFERASE; OLIGORIBONUCLEOTIDE;

ARTICLE; ATOMIC ABSORPTION SPECTROMETRY; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME IMMUNOASSAY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY; LUMINESCENCE; METABOLISM; METHODOLOGY; RADIOIMMUNOASSAY; SENSITIVITY AND SPECIFICITY; TIME;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, LIQUID; IMMUNOENZYME TECHNIQUES; LUCIFERASES; LUMINESCENT MEASUREMENTS; MOLECULAR STRUCTURE; OLIGORIBONUCLEOTIDES; RADIOIMMUNOASSAY; SENSITIVITY AND SPECIFICITY; SPECTROPHOTOMETRY, ATOMIC; TIME FACTORS;

EID: 0036009652     PISSN: 10431802     EISSN: None     Source Type: Journal    
DOI: 10.1021/bc015554l     Document Type: Article

References (16)

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7. Chemiluminescence and bioluminescence techniques
8. Selected strategies for improving sensitivity and reliability of immunoassays
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15. Inactivation of firefly luciferase with N-(iodoacetyl)-N′-(5-sulfo-1-naphthyl)ethylenediamine (I-AEDANS)
16. Enzyme immunoassay of 2′-5′-oligoadenylates at the femtomole level