A simple and rapid assay of collagen-like polymer in crude lysate from Escherichia coli

Journal of Microbiological Methods

Volumn 49, Issue 3, 2002, Pages 321-323

  Yin, Jin ^a   Tomycz, Luke ^a   Bonner, Gary ^a   Wang, Daniel I C ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

Assay; Collagen; Collagen like polymer; Collagenase; Gelatin; Ninhydrin

Indexed keywords

CLOSTRIDIOPEPTIDASE A; COLLAGEN; NINHYDRIN; POLYMER; AMINO ACID; BACTERIAL PROTEIN; COLLAGEN LIKE POLYMER; COLLAGENASE; GELATIN; UNCLASSIFIED DRUG;

ARTICLE; CHEMISTRY; COLORIMETRY; ENZYMOLOGY; ESCHERICHIA COLI; METABOLISM; ANALYTIC METHOD; ASSAY; BACTERIAL CELL; CELL LYSATE; CONTROLLED STUDY; HEAT TREATMENT; NONHUMAN; PRIORITY JOURNAL; PROTEIN DETERMINATION;

COLLAGEN; COLORIMETRY; ESCHERICHIA COLI; MICROBIAL COLLAGENASE; NINHYDRIN; POLYMERS; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, NON-P.H.S.;

ESCHERICHIA COLI;

EID: 0036008801     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(01)00372-4     Document Type: Article

References (9)

1. Modified colorimetric ninhydrin methods for peptidase assay
2. Modification of Lowry's method for collagen concentration measurement
3. Purification and assay of bacterial collagenases
4. An improved Bradford protein assay for collagen proteins
5. A modified ninhydrin colorimetric analysis for amino acids
6. Production of recombinant collagen for biomedical devices
7. Colorimetric protein assay techniques
8. Cloning and expression of collagen-derived protein in E. coli and baculovirus system