Microbial identification by immunohybridization assay of artificial RNA labels

Journal of Microbiological Methods

Volumn 49, Issue 3, 2002, Pages 301-306

  Kourentzi, Katerina D ^a   Fox, George E ^b   Willson, Richard C ^a  

^a University of Houston   (United States)

^b University of Houston   (United States)

Author keywords

Anti DNA RNA antibody; Artificial RNA; DNA probes; Hybridization; LMW RNA

Indexed keywords

ALKALINE PHOSPHATASE; DNA ANTIBODY; DNA HYBRID; RIBOSOME RNA; RNA ANTIBODY; STREPTAVIDIN; BACTERIAL RNA; HETERODUPLEX; RNA; RNA, RECOMBINANT;

ACCURACY; ANTIBODY SPECIFICITY; ARTICLE; BIOTINYLATION; CONTROLLED STUDY; DNA PROBE; DNA RNA HYBRIDIZATION; HETERODUPLEX ANALYSIS; MICROBIAL IDENTIFICATION; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; RNA SEQUENCE; BACTERIUM; ENZYME IMMUNOASSAY; ESCHERICHIA COLI; GENE VECTOR; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; NASA DISCIPLINE ENVIRONMENTAL HEALTH; NON-NASA CENTER; NUCLEIC ACID HYBRIDIZATION;

BACTERIA; BACTERIOLOGICAL TECHNIQUES; DNA PROBES; ESCHERICHIA COLI; GENETIC VECTORS; IMMUNOENZYME TECHNIQUES; NUCLEIC ACID HETERODUPLEXES; NUCLEIC ACID HYBRIDIZATION; RNA; RNA, BACTERIAL; RNA, RIBOSOMAL; STREPTAVIDIN; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, NON-P.H.S.;

NASA DISCIPLINE ENVIRONMENTAL HEALTH; NON-NASA CENTER;

EID: 0036008794     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(02)00006-4     Document Type: Article

References (13)

1. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
2. An automated fluorometric assay for alkaline phosphatase using 3-O-methylfluorescein phosphate
3. Seasonal dynamics of bacterioplankton community structure in a eutrophic lake as determined by 5S rRNA analysis
4. Microbial detection with low molecular weight RNA
5. Solution hybridization and enzyme immunoassay for biotinylated DNA-RNA hybrids to detect enteroviral RNA in cell culture
6. A novel approach for monitoring genetically engineered microorganisms by using artificial, stable RNAs
7. A low molecular weight artificial RNA of unique size with multiple probe target regions
8. Immunological capture of nucleic acid hybrids and application to nonradioactive DNA probe assay
9. Proof of hepatitis A virus negative-sense RNA by RNA/DNA-hybrid detection: A method for specific detection of both viral negative- and positive-RNA strands
10. Characterization of a Yellowstone hot spring microbial community by 5S rRNA sequences
11. Monoclonal antibody solution hybridization assay for detection of human immunodeficiency virus nucleic acids
12. A solution hybridization assay for ribosomal RNA from bacteria using biotinylated DNA probes and enzyme-labeled antibody to DNA:RNA